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Gene Review:

GPX1 - glutathione peroxidase 1

Sus scrofa

Hostetler, C.E. et al., Gross, M. et al., Lei, X.G. et al.

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High impact information on GPX1

The Se-dependent expression of two selenoproteins, cytosolic glutathione peroxidase (cGPx) and type I iodothyronine-5'-deiodinase (5'DI), was investigated in the porcine epithelial kidney cell line LLC-PK1 in serum-free medium [1].
The selenite-dependent expression of cGPx and 5'DI was revealed by enzyme-activity measurements, affinity labelling of 5'DI, metabolic labelling of proteins with 75Se and steady-state mRNA analysis [1].
Our objective was to determine dietary Se requirements of young pigs for the full expression of cellular (GPX1), plasma (GPX3) and phospholipid hydroperoxide (GPX4) glutathione peroxidases [2].
Results of this study support the hypothesis that neonatal pigs are born with reduced cGPx corresponding to reduced cGPx mRNA levels during late gestation [3].
Complementary DNA sequences encoding cGPx and TrxR-1 were cloned and sequenced [3].

Biological context of GPX1

Quantitative real-time PCR analysis indicated that levels of mRNA for cGPx in fetal liver decreased more than 3-fold between d 45 and 114 of gestation [3].
The objective of this study was to determine if levels of mRNA encoding cytosolic glutathione peroxidase (cGPx) and thioredoxin reductase (TrxR-1) change during fetal development, and if maternal Se intake during gestation affects the mRNA levels of these proteins [3].

Anatomical context of GPX1

In Experiment 1, GPX1 and GPX4 activities in liver, heart and lung were lower (P < 0.05) in pigs fed 0.1 mg Se/kg than in those fed 0.3 mg Se/kg, although no such differences existed in thyroid or pituitary [2].

Associations of GPX1 with chemical compounds

The abundant cGPx in cells with sufficient Se supply might serve as a cellular Se store which can be mobilized for the synthesis of more vital selenoproteins such as 5'DI under shortage conditions [1].

Other interactions of GPX1

Pigs fed the BD had lower (P < 0.05) tissue GPX1 and GPX4 activities, plasma GPX activity, and(or) plasma Se concentrations than those fed the Se-supplemented diets [2].

References

Differential selenium-dependent expression of type I 5'-deiodinase and glutathione peroxidase in the porcine epithelial kidney cell line LLC-PK1. Gross, M., Oertel, M., Köhrle, J. Biochem. J. (1995) [Pubmed]
Dietary selenium supplementation is required to support full expression of three selenium-dependent glutathione peroxidases in various tissues of weanling pigs. Lei, X.G., Dann, H.M., Ross, D.A., Cheng, W.H., Combs, G.F., Roneker, K.R. J. Nutr. (1998) [Pubmed]
Effect of selenium intake and fetal age on mRNA levels of two selenoproteins in porcine fetal and maternal liver. Hostetler, C.E., Michal, J., Robison, M., Ott, T.L., Kincaid, R.L. J. Anim. Sci. (2006) [Pubmed]

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