Disease relevance of CRX

• Mutations in the CRX gene are causally associated with retinal degeneration phenotypes in man [1].

High impact information on CRX

• 3. Based on RH mapping, meiotic linkage or sequencing data, we excluded CRX as the cause of canine early onset photoreceptor degenerations affecting Alaskan malamutes (cd), collies (rod-cone dysplasia 2, rcd2), American Staffordshire terriers, and Tibetan terriers [1].
• Exonic primers designed from consensus sequences of mammalian CRX cDNA were used to amplify and sequence dog genomic DNA [1].
• Canine specific primers were used for RH mapping of CRX on the RH3000 cell line [1].
• CONCLUSIONS: Canine CRX has a high level of nucleotide and amino acid sequence identity with orthologous sequences reported for other species [1].
• In agreement with human map data, RH mapping placed canine CRX on the proximal end of CFA1, in a region of synteny with HSA19q13-q13 [1].

Anatomical context of CRX

• Accordingly, we examined beta-adrenoceptor-mediated relaxations in both circumflex (CRX) and left anterior descending (LAD) coronary arteries with and without endothelium from dogs in early and end-stage heart failure induced by rapid ventricular pacing (1 and 4 weeks pacing at 250 beats x min-1, respectively) [2].

Analytical, diagnostic and therapeutic context of CRX

• To clone the full length cDNA, characterize the genomic organization of canine CRX, map the gene in a radiation hybrid (RH) panel, and evaluate it as a candidate for canine inherited retinal degenerations [1].

References