High impact information on coil

• These data on the kinetics of coilin reinforce the conclusion that CB components are in a constant state of flux, consistent with models that postulate an active role for CBs in nuclear physiology [1].
• In the Xenopus germinal vesicle (GV), most coilin actually resides in the nucleoplasm, although it is highly concentrated in 50-100 coiled bodies [2].
• Additional experiments with myc-tagged coilin and myc-tagged pyruvate kinase confirmed that coilin is a shuttling protein [2].
• Newly synthesized coilin is not required, because cycloheximide had no effect on nuclear import and subsequent targeting of the antibodies [2].
• Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes [2].

Anatomical context of coil

• Coiled bodies without coilin [3].
• Because the nuclear envelope does not allow passive diffusion of immunoglobulins, this observation suggests that anti-coilin antibodies are imported into the nucleus as an antigen-antibody complex with coilin [2].
• Each of these organelles contains coilin or a coilin-related protein plus a variety of small nuclear ribonucleoproteins [4].

Analytical, diagnostic and therapeutic context of coil

• We labeled coilin with photoactivatable green fluorescent protein and analyzed the movement of the protein by confocal microscopy in unfixed germinal vesicles isolated in oil [1].
• Because coilin is not detected in the cytoplasm by immunofluorescence and Western blotting, it has been considered an exclusively nuclear protein [2].
• To determine whether coilin is an essential structural component of the coiled body, we removed it from the egg extract by immunoprecipitation [3].

References