High impact information on NUSAP1

• Direct interaction of NuSAP with microtubules was demonstrated in vitro [1].
• In addition, many NuSAP-depleted interphase cells had deformed nuclei [1].
• Both overexpression and knockdown of NuSAP impaired cell proliferation [1].
• In contrast, depletion of NuSAP by RNA interference resulted in aberrant mitotic spindles, defective chromosome segregation, and cytokinesis [1].
• Microscopic analysis of both fixed and live mammalian cells showed that NuSAP is primarily nucleolar in interphase, and localizes prominently to central spindle microtubules during mitosis [1].

Biological context of NUSAP1

• We propose that the presence of MT binding agents such as NuSAP, which can be directly immobilized on chromatin, are critical for targeting MT production to vertebrate chromosomes during spindle self-organization [2].
• Each mutagenic codon cassette contains a three base pair direct terminal repeat and two head-to-head recognition sequences for the restriction endonuclease Sapl, an enzyme that cleaves outside of its recognition sequence [3].

Anatomical context of NUSAP1

• These results suggest a crucial role for NuSAP in spindle microtubule organization [1].

Associations of NUSAP1 with chemical compounds

• Only ritonavir and saquinavir inhibited Sap2p, Sapt1p, Sapp1p, and Saplp in micromolar concentrations [4].

Other interactions of NUSAP1

• RanGTP is generated at chromatin and activates a set of soluble, Ran-regulated spindle factors such as TPX2, NuMA, and NuSAP . How the spindle factors direct and attach MTs to the chromosomes are key open questions [2].

References