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Gene Review:

PMP22 - peripheral myelin protein 22

Bos taurus

Kitamura, K. et al., Sedzik, J. et al., Sedzik, J. et al., Uyemura, K. et al.

Kitamura, Uyemura, Shibuya, Sakamoto, Yoshimura, Nomura, Sedzik, Uyemura, Tsukihara,

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High impact information on LOC534497

The amino acid sequence of the glycopeptide obtained from bovine PASII/PMP22 protein in the PNS myelin was determined to be Gln-Asn-Cys-Ser-Thr, where the asparagine was glycosylated [1].
Structure of a major oligosaccharide of PASII/PMP22 glycoprotein in bovine peripheral nerve myelin [1].
We usually obtained 10-20 mg PASII/PMP22 from 10 g bovine spinal roots [2].
Human PASII/PMP22 sequence was deduced and the locus of its gene, chromosome 17p12-p11.2, is similar to the region linked to Charcot-Marie-Tooth disease type 1A [3].

Associations of LOC534497 with chemical compounds

We solubilized myelin membrane in 2%(w/v) sodium dodecyl sulfate, followed by chromatographic purification of the hydrophobic myelin glycoproteins P0 and PASII/PMP22, and finally, we have exchanged the sodium dodecyl sulfate bound to protein for other neutral detergents using ceramic hydroxyapatite column [4].

Analytical, diagnostic and therapeutic context of LOC534497

The labeled PASII/PMP22 glycopeptides were isolated by HPLC and were digested further with glycopeptidase A [1].
Here we describe a simple method for purification of PASII/PMP22, suitable for crystallization trials [2].

References

Structure of a major oligosaccharide of PASII/PMP22 glycoprotein in bovine peripheral nerve myelin. Kitamura, K., Uyemura, K., Shibuya, K., Sakamoto, Y., Yoshimura, K., Nomura, M. J. Neurochem. (2000) [Pubmed]
Purification of PASII/PMP22--an extremely hydrophobic glycoprotein of PNS myelin membrane. Sedzik, J., Kotake, Y., Uyemura, K. Neuroreport (1998) [Pubmed]
Structure and function of peripheral nerve myelin proteins. Uyemura, K., Asou, H., Takeda, Y. Prog. Brain Res. (1995) [Pubmed]
Towards crystallization of hydrophobic myelin glycoproteins: P0 and PASII/PMP22. Sedzik, J., Uyemura, K., Tsukihara, T. Protein Expr. Purif. (2002) [Pubmed]

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PMP22	Canis lupus familiaris
pmp22b	Danio rerio
PMP22	Gallus gallus
PMP22	Homo sapiens
PMP22	Macaca mulatta
Pmp22	Mus musculus
PMP22	Pan troglodytes
Pmp22	Rattus norvegicus
pmp22	Xenopus (Silurana) tropicalis

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