High impact information on LOC548237

• The promoter of the barley aleurone-specific gene encoding a putative 7 kDa lipid transfer protein confers aleurone cell-specific expression in transgenic rice [1].
• Identification of an enhancer/silencer sequence directing the aleurone-specific expression of a barley chitinase gene [2].
• The first type, which contained two copies of the E-region fused to the CaMV 35S TATA box, conferred aleurone-specific expression of a GUS reporter gene [2].
• Transient gene expression assays have been used in tissues of barley to delineate promoter sequences involved in the regulation of an aleurone-specific chitinase gene (Chi26), and of a vegetatively expressed chitinase gene (Chi33) [2].
• One of these clones, p48h-10, shows high protein sequence homology to a barley aleurone-specific cDNA, B11E [3].

Biological context of LOC548237

• A homology search of the nucleotide and amino acid sequences of TED4 with several data bases revealed a significant similarity to those of the barley aleurone-specific clone B11E, which was isolated as an aleurone-specific cDNA from 20-d postanthesis grain [4].
• From the deletion studies, a 49-bp promoter region directing aleurone-specific expression was identified [5].
• Silencing of the aleurone-specific Ltp2-gus gene in transgenic rice is reversed by transgene rearrangements and loss of aberrant transcripts [6].
• Previously, we reported the isolation of silenced plants in the R2 generation and the involvement of antisense gus transcripts in aleurone-specific Ltp2-gus gene silencing in transgenic rice, the L3.3 line [6].

Analytical, diagnostic and therapeutic context of LOC548237

• An aleurone-specific AGP was detected by reversed-phase HPLC, and supported the idea that an AGP may play an important role in aleurone-specific events [7].

References