Disease relevance of PPP1R2

• Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli [1].
• Cloning of a cDNA encoding an isoform of human protein phosphatase inhibitor 2 from vascularized breast tumor [2].

High impact information on PPP1R2

• Activation of Aurora-A kinase by protein phosphatase inhibitor-2, a bifunctional signaling protein [3].
• Here, we describe the cloning and characterization of a novel human transmembrane protein KPI-2 (Kinase/Phosphatase/Inhibitor-2) that was identified by yeast two-hybrid using protein phosphatase inhibitor-2 (Inh2) as bait [4].
• The previously reported homologue of PPP1R2 on chromosome 5 is identified as an intronless pseudogene [5].
• This study investigates whether genetic alteration(s) in the I-2 coding locus (PPP1R2) could contribute to insulin resistance in Pima Indians [5].
• We determined that the authentic PPP1R2 gene is located on chromosome 3q29 and consists of six exons [5].

Biological context of PPP1R2

• Southern hybridization results were consistent with the existence of a family of related IPP-2 sequences in the human genome [6].
• The human nucleotide sequence was highly homologous (94% identity) to the rabbit IPP-2 sequence and encoded a peptide of 205 amino acids [6].
• By in situ hybridization IPP-2 sequences were mapped to several different human chromosomes [6].
• We sequenced one gene located in the major histocompatibility complex (MHC) on Chromosome (Chr) 6 that contained the entire coding region of IPP-2 [6].
• Amino acid sequence at the site on protein phosphatase inhibitor-2, phosphorylated by glycogen synthase kinase-3 [7].

Other interactions of PPP1R2

• The information on the genomic structure of PPP1R2 is important for exploring this gene as a potential candidate contributing to insulin resistance and NIDDM in other populations [5].

References