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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

CHST7  -  carbohydrate (N-acetylglucosamine 6-O)...

Homo sapiens

Synonyms: C6ST-2, C6ST2, Carbohydrate sulfotransferase 7, Chondroitin 6-sulfotransferase 2, GST-5, ...
 
 
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Disease relevance of CHST7

  • N-acetylglucosamine-6-O-sulfotransferase-1: production in the baculovirus system and its applications to the synthesis of a sulfated oligosaccharide and to the modification of oligosaccharides in fibrinogen [1].
 

High impact information on CHST7

  • To ascertain the importance of the carbohydrate chain sulfation step in KS functionality, we generated a strain of mice with a targeted gene deletion in Chst5, which encodes an N-acetylglucosamine-6-O-sulfotransferase that is integral to the sulfation of KS chains [2].
  • The identification of the reaction product indicated that the enzyme is a novel chondroitin 6-O-sulfotransferase (C6ST-2) that mainly transfers sulfate to N-acetylgalactosamine [3].
  • The coding region of C6ST-2 was contained in a single exon and localized to chromosome Xp11 [3].
  • In particular, GST-5 was found to catalyze 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc [4].
  • Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5) [4].
 

Anatomical context of CHST7

  • We have coexpressed a human GST-5 cDNA with a GlyCAM-1/IgG fusion protein in COS-7 cells and observed four-fold enhanced [(35)S]sulfate incorporation into this mucin acceptor [4].
  • Characterization of an N-acetylglucosamine-6-O-sulfotransferase from human respiratory mucosa active on mucin carbohydrate chains [5].
 

Associations of CHST7 with chemical compounds

 

Analytical, diagnostic and therapeutic context of CHST7

  • Furthermore, we have applied the HPLC data to identification of isomeric products resulting from an enzymatic reaction of N-acetylglucosamine 6-O-sulfotransferase-1 in vitro and revealed that this enzyme preferentially catalyzes sulfation of the GlcNAcbeta1-->2Manalpha1-->3Man branch in a biantennary acceptor [7].
  • Using an enzyme-linked immunosorbent assay (ELISA) and Western blot analysis, both anti-GST-5 sera and the monoclonal antibody were shown to react with GST-5 [8].

References

  1. N-acetylglucosamine-6-O-sulfotransferase-1: production in the baculovirus system and its applications to the synthesis of a sulfated oligosaccharide and to the modification of oligosaccharides in fibrinogen. El-Fasakhany, F.M., Ichihara-Tanaka, K., Uchimura, K., Muramatsu, T. J. Biochem. (2003) [Pubmed]
  2. Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. Hayashida, Y., Akama, T.O., Beecher, N., Lewis, P., Young, R.D., Meek, K.M., Kerr, B., Hughes, C.E., Caterson, B., Tanigami, A., Nakayama, J., Fukada, M.N., Tano, Y., Nishida, K., Quantock, A.J. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  3. Molecular cloning and expression of a novel chondroitin 6-O-sulfotransferase. Kitagawa, H., Fujita, M., Ito, N., Sugahara, K. J. Biol. Chem. (2000) [Pubmed]
  4. Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5). Bhakta, S., Bartes, A., Bowman, K.G., Kao, W.M., Polsky, I., Lee, J.K., Cook, B.N., Bruehl, R.E., Rosen, S.D., Bertozzi, C.R., Hemmerich, S. J. Biol. Chem. (2000) [Pubmed]
  5. Characterization of an N-acetylglucosamine-6-O-sulfotransferase from human respiratory mucosa active on mucin carbohydrate chains. Degroote, S., Lo-Guidice, J.M., Strecker, G., Ducourouble, M.P., Roussel, P., Lamblin, G. J. Biol. Chem. (1997) [Pubmed]
  6. Diversity of N-acetylglucosamine-6-O-sulfotransferases: molecular cloning of a novel enzyme with different distribution and specificities. Uchimura, K., Fasakhany, F., Kadomatsu, K., Matsukawa, T., Yamakawa, T., Kurosawa, N., Muramatsu, T. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  7. Development of structural analysis of sulfated N-glycans by multidimensional high performance liquid chromatography mapping methods. Yagi, H., Takahashi, N., Yamaguchi, Y., Kimura, N., Uchimura, K., Kannagi, R., Kato, K. Glycobiology (2005) [Pubmed]
  8. Epitope analysis of a sperm acrosomal antigen defined by HSA-5 monoclonal antibody. Lee, C.Y., Yoshiki, T., Chui, L., McChesney, P., Herr, J.C., Hwang, E.S., Huang, E.S. J. Reprod. Immunol. (1995) [Pubmed]
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