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Gene Review

SPC25  -  SPC25, NDC80 kinetochore complex component

Homo sapiens

Synonyms: AD024, Kinetochore protein Spc25, MGC22228, SPBC25, hSpc25
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High impact information on SPC25

  • Formation of the complete KMN network, which additionally requires the Mis12 complex and the Spc24/Spc25 subunits of the Ndc80 complex, synergistically enhances microtubule-binding activity [1].
  • Although the sequences have greatly diverged, we have concluded that these are the functional homologs of the yeast Spc24 and Spc25 proteins based on limited sequence similarity, common coiled-coil domains, kinetochore localization, similar phenotypes, and copurification with xNdc80 and xNuf2 [2].
  • In addition, we show that Spc24 and Spc25 are required for chromosomal movement to the spindle poles in anaphase [2].
  • Using both RNAi and antibody injection experiments, we have extended previous characterization of the complex and found that Spc24 and Spc25 are required not only to establish, but also to maintain kinetochore-microtubule attachments and metaphase alignment [2].

Other interactions of SPC25

  • Two tight Hec1-Nuf2 and Spc24-Spc25 subcomplexes, each stabilized by a parallel heterodimeric coiled coil, maintain this organization [3].

Analytical, diagnostic and therapeutic context of SPC25

  • We report the atomic structures of the Spc24/Spc25 globular domain, determined both by X-ray crystallography at 1.9 A resolution and by NMR [4].


  1. The Conserved KMN Network Constitutes the Core Microtubule-Binding Site of the Kinetochore. Cheeseman, I.M., Chappie, J.S., Wilson-Kubalek, E.M., Desai, A. Cell (2006) [Pubmed]
  2. The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment. McCleland, M.L., Kallio, M.J., Barrett-Wilt, G.A., Kestner, C.A., Shabanowitz, J., Hunt, D.F., Gorbsky, G.J., Stukenberg, P.T. Curr. Biol. (2004) [Pubmed]
  3. Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. Ciferri, C., De Luca, J., Monzani, S., Ferrari, K.J., Ristic, D., Wyman, C., Stark, H., Kilmartin, J., Salmon, E.D., Musacchio, A. J. Biol. Chem. (2005) [Pubmed]
  4. Structure of a central component of the yeast kinetochore: the Spc24p/Spc25p globular domain. Wei, R.R., Schnell, J.R., Larsen, N.A., Sorger, P.K., Chou, J.J., Harrison, S.C. Structure (2006) [Pubmed]
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