High impact information on PCDHB16

• Northern blot analysis revealed that 1.3kb ME1 mRNA is highly expressed in the mouse epididymis [1].
• The deduced amino-acid sequence of the ME1 cDNA revealed that it consists of 149 amino acid residues, which contain a signal peptide characteristic of secretory proteins, six cysteine residues and a proline-rich region conserved in the orthologous proteins [1].
• The reduction of the molecular mass of the recombinant ME1 protein with the digestion of glycopeptidase A indicated that it is modified by Asn-linked glycosylation [1].

Anatomical context of PCDHB16

• Primary structure, genomic organization and expression of the major secretory protein of murine epididymis, ME1 [1].

Analytical, diagnostic and therapeutic context of PCDHB16

• Out of the 32 residues comprising the transmembrane helix of GpA, five amino acids were mutated; the resulting protein, ME1, has been characterized in dodecyl phosphocholin (DPC) micelles by UV-vis, CD spectroscopy, gel electrophoresis, and analytical ultracentrifugation [2].
• The expression of ME1 forms was found to be absent in B*2704 N97D by immunoprecipitation and flow cytometry of fixed and permeabilized cell experiments with the conformation-sensitive ME1 antibody [3].

References