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MeSH Review


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Disease relevance of Ultracentrifugation


High impact information on Ultracentrifugation

  • The distribution of the HDL subfractions HDL2, HDL3, and HDL3D, as determined by zonal ultracentrifugation, was normal in black and abnormal in white men receiving hemodialysis [6].
  • We show that after reaction with DNA bearing Chi sites, RecBCD enzyme is inactivated and the three subunits migrate as separate species during glycerol gradient ultracentrifugation or native gel electrophoresis [7].
  • Sucrose density gradient ultracentrifugation studies of serum samples of patients with EM after HSV infection showed HSV antigen in large molecular weight fractions [8].
  • Analyses of the wild-type (wt) and mutant STAT1 proteins by static light scattering, analytical ultracentrifugation, and coimmunoprecipitation suggest that STAT1 is predominantly dimeric prior to activation, and the dimer is mediated by the ND interactions [9].
  • Masked TLF1 activity in the column fractions is revealed if Hp is removed by density gradient ultracentrifugation [10].

Chemical compound and disease context of Ultracentrifugation


Biological context of Ultracentrifugation


Anatomical context of Ultracentrifugation


Associations of Ultracentrifugation with chemical compounds

  • Rate zonal ultracentrifugation of CER-VLDL from rabbits fed cholesterol for 4 and 60 d demonstrated an early increase of the proportion of cholesterol carried in the intestinally-derived fraction (designated as Fx-I) of VLDL compared with that in hepatically-derived particles (Fx-H) [26].
  • No HDL could be detected by analytic ultracentrifugation, but polyacrylamide gradient gel electrophoresis (gge) revealed that patients possessed two major HDL subclasses: (HDL2b)gge at 11.0 nm and (HDL3b)gge at 7.8 nm [27].
  • Analytical and zonal ultracentrifugation revealed a marked increase in triglyceride-rich lipoproteins including chylomicrons and very low density lipoproteins, a reduction in LDL, and the presence of virtually only the HDL3 subfraction [28].
  • Colonic mucins were purified from uninvolved surgical specimens by gel filtration with Sepharose 4B and cesium chloride-guanidine hydrochloride density gradient ultracentrifugation [29].
  • Phosphorylated proteins and PhGlc colocalized in the Triton X-100 insoluble, light buoyant density fraction after sucrose gradient ultracentrifugation of HL60 cell lysates [30].

Gene context of Ultracentrifugation

  • Comparative lipid-binding studies with dimyristoyl phosphatidylcholine gave complexes for native and synthetic apoprotein which floated at the same density after ultracentrifugation in KBr gradients and had virtually the same lipid:protein ratios [31].
  • When RBC of increasing age was separated by buoyant density ultracentrifugation, the total HK activity decayed in a biphasic manner, with half-lives respectively of approximately 15 and approximately 51 days [32].
  • Furthermore, platelet VN and PAI-1 in Ca-ionophore releasates comigrated during ultracentrifugation in high mol wt fractions of sucrose density gradients, indicating a possible association of both components [33].
  • Analytical ultracentrifugation experiments showed that purified Mlh1p forms a homodimer in solution, albeit with a K(d) of 3.14 microM, 36-fold higher than that for Mlh1p-Pms1p heterodimerization [34].
  • Mutants deficient in their ability to activate LCAT displayed alterations in liposome and HDL binding, compared with WT as determined by density gradient ultracentrifugation analysis of the culture medium [35].

Analytical, diagnostic and therapeutic context of Ultracentrifugation


  1. Molecular characterization of circulating EBV DNA in the plasma of nasopharyngeal carcinoma and lymphoma patients. Chan, K.C., Zhang, J., Chan, A.T., Lei, K.I., Leung, S.F., Chan, L.Y., Chow, K.C., Lo, Y.M. Cancer Res. (2003) [Pubmed]
  2. Subunit structure of biodegradative threonine deaminase. Saeki, Y., Ito, S., Shizuta, Y., Hayaishi, O., Kagamiyama, H., Wada, H. J. Biol. Chem. (1977) [Pubmed]
  3. Characterization of NADH-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from Pseudomonas arvilla c-1. Yamaguchi, M., Fujisawa, H. J. Biol. Chem. (1978) [Pubmed]
  4. Serum IgM-bound secretory component (sIgM) in liver diseases: comparative molecular state of the secretory component in serum and bile. Delacroix, D.L., Jonard, P., Dive, C., Vaerman, J.P. J. Immunol. (1982) [Pubmed]
  5. Isolation and characterization of circulating feline leukemia virus-immune complexes from plasma of persistently infected pet cats removed by ex vivo immunosorption. Snyder, H.W., Jones, F.R., Day, N.K., Hardy, W.D. J. Immunol. (1982) [Pubmed]
  6. Racial differences in plasma high-density lipoproteins in patients receiving hemodialysis. A possible mechanism for accelerated atherosclerosis in white men. Goldberg, A.P., Harter, H.R., Patsch, W., Schechtman, K.B., Province, M., Weerts, C., Kuisk, I., McCrate, M.M., Schonfeld, G. N. Engl. J. Med. (1983) [Pubmed]
  7. Regulation of homologous recombination: Chi inactivates RecBCD enzyme by disassembly of the three subunits. Taylor, A.F., Smith, G.R. Genes Dev. (1999) [Pubmed]
  8. Herpes simplex antigen in immune complexes of patients with erythema multiforme: presence following recurrent herpes simplex infection. Kazmierowski, J.A., Peizner, D.S., Wuepper, K.D. JAMA (1982) [Pubmed]
  9. Structural bases of unphosphorylated STAT1 association and receptor binding. Mao, X., Ren, Z., Parker, G.N., Sondermann, H., Pastorello, M.A., Wang, W., McMurray, J.S., Demeler, B., Darnell, J.E., Chen, X. Mol. Cell (2005) [Pubmed]
  10. The main lytic factor of Trypanosoma brucei brucei in normal human serum is not high density lipoprotein. Raper, J., Nussenzweig, V., Tomlinson, S. J. Exp. Med. (1996) [Pubmed]
  11. Purification and properties of ornithine decarboxylase from Lactobacillus sp. 30a. Guirard, B.M., Snell, E.E. J. Biol. Chem. (1980) [Pubmed]
  12. Increased small dense LDL and intermediate-density lipoprotein with albuminuria in type 1 diabetes. Sibley, S.D., Hokanson, J.E., Steffes, M.W., Purnell, J.Q., Marcovina, S.M., Cleary, P.A., Brunzell, J.D. Diabetes Care (1999) [Pubmed]
  13. Size-exclusion chromatography purification of high-titer vesicular stomatitis virus G glycoprotein-pseudotyped retrovectors for cell and gene therapy applications. Transfiguracion, J., Jaalouk, D.E., Ghani, K., Galipeau, J., Kamen, A. Hum. Gene Ther. (2003) [Pubmed]
  14. Human breast tumor estrogen receptor: effects of molybdate and electrophoretic analyses. Miller, L.K., Tuazon, F.B., Niu, E.M., Sherman, M.R. Endocrinology (1981) [Pubmed]
  15. Estrogen receptor cleavage and plasminogen activation by enzymes in human breast tumor cytosol. Sherman, M.R., Tuazon, F.B., Miller, L.K. Endocrinology (1980) [Pubmed]
  16. Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes. Sheets, E.D., Holowka, D., Baird, B. J. Cell Biol. (1999) [Pubmed]
  17. In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha. Marchesi, V.T., Ngo, N. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  18. Molecular cloning of the cDNA for stimulatory GDP/GTP exchange protein for smg p21s (ras p21-like small GTP-binding proteins) and characterization of stimulatory GDP/GTP exchange protein. Kaibuchi, K., Mizuno, T., Fujioka, H., Yamamoto, T., Kishi, K., Fukumoto, Y., Hori, Y., Takai, Y. Mol. Cell. Biol. (1991) [Pubmed]
  19. Characterization of the human plasma binding protein for vitamin D and its metabolites synthesized by the human hepatoma-derived cell line, Hep 3B. Haddad, J.G., Aden, D.P., Kowalski, M.A. J. Biol. Chem. (1983) [Pubmed]
  20. 25-Hydroxyvitamin D transport in human plasma. Isolation and partial characterization of calcifidiol-binding protein. Haddad, J.G., Walgate, J. J. Biol. Chem. (1976) [Pubmed]
  21. Chemical nature of alcoholic hyalin. Luisada-Opper, A.V., Kanagasundaram, N., Leevy, C.M. Gastroenterology (1977) [Pubmed]
  22. Immunological characterization of a basement membrane-specific chondroitin sulfate proteoglycan. McCarthy, K.J., Accavitti, M.A., Couchman, J.R. J. Cell Biol. (1989) [Pubmed]
  23. Choriocarcinoma with hyperthyroidism: probable identity of the thyrotropin with human chorionic gonadotropin. Cave, W.T., Dunn, J.T. Ann. Intern. Med. (1976) [Pubmed]
  24. Physicochemical and genetic evidence for specific antiestrogen binding sites. Faye, J.C., Jozan, S., Redeuilh, G., Baulieu, E.E., Bayard, F. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  25. Identification of endothelin 1 and big endothelin 1 in secretory vesicles isolated from bovine aortic endothelial cells. Harrison, V.J., Barnes, K., Turner, A.J., Wood, E., Corder, R., Vane, J.R. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  26. Dependence of metabolic and structural heterogeneity of cholesterol ester-rich very low density lipoproteins on the duration of cholesterol feeding in rabbits. Daugherty, A., Oida, K., Sobel, B.E., Schonfeld, G. J. Clin. Invest. (1988) [Pubmed]
  27. Familial apolipoprotein AI and apolipoprotein CIII deficiency. Subclass distribution, composition, and morphology of lipoproteins in a disorder associated with premature atherosclerosis. Forte, T.M., Nichols, A.V., Krauss, R.M., Norum, R.A. J. Clin. Invest. (1984) [Pubmed]
  28. Apolipoprotein C-II deficiency syndrome. Clinical features, lipoprotein characterization, lipase activity, and correction of hypertriglyceridemia after apolipoprotein C-II administration in two affected patients. Baggio, G., Manzato, E., Gabelli, C., Fellin, R., Martini, S., Enzi, G.B., Verlato, F., Baiocchi, M.R., Sprecher, D.L., Kashyap, M.L. J. Clin. Invest. (1986) [Pubmed]
  29. Aberrant expression of monoclonal antibody-defined colonic mucosal antigens in inflammatory bowel disease. Haviland, A.E., Borowitz, M.J., Lan, M.S., Kaufman, B., Khorrami, A., Phelps, P.C., Metzgar, R.S. Gastroenterology (1988) [Pubmed]
  30. Carbohydrate-dependent signaling from the phosphatidylglucoside-based microdomain induces granulocytic differentiation of HL60 cells. Nagatsuka, Y., Hara-Yokoyama, M., Kasama, T., Takekoshi, M., Maeda, F., Ihara, S., Fujiwara, S., Ohshima, E., Ishii, K., Kobayashi, T., Shimizu, K., Hirabayashi, Y. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  31. The solid phase synthesis of a protein activator for lecithin-cholesterol acyltransferase corresponding to human plasma apoC-I. Sigler, G.F., Soutar, A.K., Smith, L.C., Gotto, A.M., Sparrow, J.T. Proc. Natl. Acad. Sci. U.S.A. (1976) [Pubmed]
  32. An isozyme of hexokinase specific for the human red blood cell (HKR). Murakami, K., Blei, F., Tilton, W., Seaman, C., Piomelli, S. Blood (1990) [Pubmed]
  33. Identification of and partial characterization of platelet vitronectin: evidence for complex formation with platelet-derived plasminogen activator inhibitor-1. Preissner, K.T., Holzhüter, S., Justus, C., Müller-Berghaus, G. Blood (1989) [Pubmed]
  34. Inactivation of DNA mismatch repair by increased expression of yeast MLH1. Shcherbakova, P.V., Hall, M.C., Lewis, M.S., Bennett, S.E., Martin, K.J., Bushel, P.R., Afshari, C.A., Kunkel, T.A. Mol. Cell. Biol. (2001) [Pubmed]
  35. Site-directed mutagenesis and structure-function analysis of the human apolipoprotein A-I. Relation between lecithin-cholesterol acyltransferase activation and lipid binding. Minnich, A., Collet, X., Roghani, A., Cladaras, C., Hamilton, R.L., Fielding, C.J., Zannis, V.I. J. Biol. Chem. (1992) [Pubmed]
  36. Membrane attack complex of complement: a structural analysis of its assembly. Podack, E.R., Esser, A.F., Biesecker, G., Müller-Eberhard, H.J. J. Exp. Med. (1980) [Pubmed]
  37. The immunopathology of herpes gestationis. Immunofluorescence studies and characterization of "HG factor". Jordon, R.E., Heine, K.G., Tappeiner, G., Bushkell, L.L., Provost, T.T. J. Clin. Invest. (1976) [Pubmed]
  38. Expression of chicken lamin B2 in Escherichia coli: characterization of its structure, assembly, and molecular interactions. Heitlinger, E., Peter, M., Häner, M., Lustig, A., Aebi, U., Nigg, E.A. J. Cell Biol. (1991) [Pubmed]
  39. Role of carbohydrate in multimeric structure of factor VIII/von Willebrand factor protein. Gralnick, H.R., Williams, S.B., Rick, M.E. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  40. Inverse relationship between blood levels of high density lipoprotein subfraction 2 and magnitude of postprandial lipemia. Patsch, J.R., Karlin, J.B., Scott, L.W., Smith, L.C., Gotto, A.M. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
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