Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

FAM110D - family with sequence similarity 110, member D

Homo sapiens

Synonyms: FLJ14050, GRRP1, Protein FAM110D

Mukohara, T. et al., Tang, J. et al., Boisvert, F.M. et al., Kim, K. et al., de Cárcer, G. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on GRRP1

Complex formation in vitro and in vivo requires a 63-residue glycine-arginine-rich (GAR) domain located at the extreme C terminus of nucleolin, with this domain sufficient to inhibit DNA replication in vitro [1].
Although asymmetric NG,NG-dimethylation of arginine residues in glycine-arginine-rich domains of eucaryotic proteins, catalyzed by type I protein arginine N-methyltransferases (PRMT), has been known for some time, members of this enzyme class have only recently been cloned [2].
METHODS: NSCLC cell lines carrying wild-type EGFR (A549, H441, and H1666) or mutant EGFR (H3255, DFCILU-011, PC-9, and HCC827) were treated with various dilutions of gefitinib or cetuximab relative to maximal achievable serum concentration [3].
53BP1 contains a glycine arginine rich (GAR) motif of unknown function within its kinetochore-binding domain [4].
Our findings identify the GAR motif as a region required for 53BP1 DNA binding activity and as the site of methylation by PRMT1 [4].

Biological context of GRRP1

Both agents also induced no (H441) or moderate (H1666) apoptosis in NSCLC cells with wild-type EGFR [3].

Associations of GRRP1 with chemical compounds

RESULTS: Growth of NSCLC lines with wild-type EGFR was slightly (A549 and H441) or moderately (H1666) inhibited by gefitinib and cetuximab, and the effects of the two agents were similar [3].

Physical interactions of GRRP1

In mammals, the highest homology was with 50-kDa nucleolin fragments containing the RNA-binding motifs and the glycine-arginine-rich (GAR) domain [5].

Analytical, diagnostic and therapeutic context of GRRP1

Deletion and site-specific mutagenesis of nucleolin's carboxy GAR domain [6].

References

Novel checkpoint response to genotoxic stress mediated by nucleolin-replication protein a complex formation. Kim, K., Dimitrova, D.D., Carta, K.M., Saxena, A., Daras, M., Borowiec, J.A. Mol. Cell. Biol. (2005) [Pubmed]
PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation. Tang, J., Gary, J.D., Clarke, S., Herschman, H.R. J. Biol. Chem. (1998) [Pubmed]
Differential effects of gefitinib and cetuximab on non-small-cell lung cancers bearing epidermal growth factor receptor mutations. Mukohara, T., Engelman, J.A., Hanna, N.H., Yeap, B.Y., Kobayashi, S., Lindeman, N., Halmos, B., Pearlberg, J., Tsuchihashi, Z., Cantley, L.C., Tenen, D.G., Johnson, B.E., Jänne, P.A. J. Natl. Cancer Inst. (2005) [Pubmed]
The GAR motif of 53BP1 is arginine methylated by PRMT1 and is necessary for 53BP1 DNA binding activity. Boisvert, F.M., Rhie, A., Richard, S., Doherty, A.J. Cell Cycle (2005) [Pubmed]
NopA64, a novel nucleolar phosphoprotein from proliferating onion cells, sharing immunological determinants with mammalian nucleolin. de Cárcer, G., Cerdido, A., Medina, F.J. Planta (1997) [Pubmed]
Deletion and site-specific mutagenesis of nucleolin's carboxy GAR domain. Pellar, G.J., DiMario, P.J. Chromosoma (2003) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

FAM110D	Bos taurus
FAM110D	Canis lupus familiaris
si:ch211-107n13.1	Danio rerio
FAM110D	Gallus gallus
LOC719783	Macaca mulatta
Grrp1	Mus musculus
FAM110D	Pan troglodytes
Fam110d	Rattus norvegicus

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.