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Gene Review:

SUP53 - tRNA

Saccharomyces cerevisiae S288c

Fischhoff, D.A. et al., Strobel, M.C. et al., Liebman, S.W. et al., Newman, A.J. et al.

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High impact information on SUP53

The yeast SUP53 gene encodes a leucine-inserting amber suppressor tRNA [1].
Intron mutations affect splicing of Saccharomyces cerevisiae SUP53 precursor tRNA [2].
The in vitro phenotype of these mutant RNAs was correlated with the in vivo suppressor tRNA function of these SUP53 alleles after integration of the genes into the yeast genome [2].
We show by protein analysis that like SUP52, the suppressors SUP53 through SUP56 are leucine-inserters [3].
We have taken advantage of the correlation between the suppressor mutation and the XbaI site formation, to show that the tRNALeu3 gene on YEp13 corresponds to the genetically characterized yeast chromosomal amber suppressor SUP53 . We have also shown that SUP53 is located just centromere-distal to LEU2 on chromosome III [4].

Biological context of SUP53

Finally, comparison of the DNA sequence of SUP53 and its flanking regions with the sequences of other cloned yeast tRNALeu3 genes has revealed considerable sequence homology in the immediate 5'-flanking regions of these genes [4].

References

tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter. Newman, A.J., Ogden, R.C., Abelson, J. Cell (1983) [Pubmed]
Intron mutations affect splicing of Saccharomyces cerevisiae SUP53 precursor tRNA. Strobel, M.C., Abelson, J. Mol. Cell. Biol. (1986) [Pubmed]
Yeast amber suppressors corresponding to tRNA3Leu genes. Liebman, S.W., Srodulski, Z., Reed, C.R., Stewart, J.W., Sherman, F., Brennan, G. J. Mol. Biol. (1984) [Pubmed]
The yeast cloning vector YEp13 contains a tRNALeu3 gene that can mutate to an amber suppressor. Fischhoff, D.A., Waterston, R.H., Olson, M.V. Gene (1984) [Pubmed]

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