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Gene Review:

GBP2 - Gbp2p

Saccharomyces cerevisiae S288c

Synonyms: G-strand-binding protein 2, RAP1 localization factor 6, RLF6, Single-strand telomeric DNA-binding protein GBP2, YCL011C, ...

Windgassen, M. et al., Hurt, E. et al., Konkel, L.M. et al., Pang, T.L. et al., Lin, J.J. et al., et al.

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High impact information on GBP2

Consistent with these findings, Gbp2 and Hrb1 associate with actively transcribed genes throughout their entire lengths [1].
We also show that Gbp2 and Hrb1 interact with Ctk1, a kinase that phosphorylates the C-terminal domain of RNA polymerase II during transcription elongation [1].
In addition, expression of Chlamydomonas GBP1 restores perinuclear, punctate Rap1p localization in rlf6 mutant cells [2].
The functional complementation of a fungal gene by an algal gene suggests that Rlf6p and Gbp1p are members of a conserved class of single-stranded telomeric DNA-binding proteins that influence nuclear organization [2].
The protein is similar to the other two serine/arginine (SR)-type proteins in yeast, Gbp2 and Npl3 [3].

Biological context of GBP2

Although GBP2 is not essential for normal cell growth, overexpression of this gene is toxic and causes a nuclear retention of bulk poly(A)(+) RNA [4].
The second gene, GBP2 (G-strand Binding Protein), is an anonymous open reading frame from chromosome III [5].
The nuclear export of Gbp2 is always coupled to mRNA export and is dependent on continuous RNA polymerase II transcription and mRNA-export factors [4].
Moreover, the binding activity of Gbp2p to single-stranded TG(1-3) DNA appears to be associated with its ability to restore the telomere-lengthening phenotype in cdc13-1 cells [6].
These results indicate that Gbp2p is involved in modulating telomere length [6].

Associations of GBP2 with chemical compounds

Deletion of the genes encoding both of these proteins or disruption of two of the arginine/serine repeats each shifts the steady-state localization of Gbp2 to the cytoplasm [4].

Other interactions of GBP2

Nuclear import of Gbp2 is dependent on the receptor Mtr10 and the serine/arginine-specific protein kinase Sky1 [4].
Both Nsr1p and Gbp2p bind specifically to yeast single strand (TG1-3)n DNA in vitro [5].

Analytical, diagnostic and therapeutic context of GBP2

By using an RNA immunoprecipitation assay, we show that Gbp2 and Hrb1 also are bound to transcripts that are derived from these genes [1].

References

Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. Hurt, E., Luo, M.J., Röther, S., Reed, R., Strässer, K. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
A class of single-stranded telomeric DNA-binding proteins required for Rap1p localization in yeast nuclei. Konkel, L.M., Enomoto, S., Chamberlain, E.M., McCune-Zierath, P., Iyadurai, S.J., Berman, J. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
Differential export requirements for shuttling serine/arginine-type mRNA-binding proteins. Häcker, S., Krebber, H. J. Biol. Chem. (2004) [Pubmed]
Identification of Gbp2 as a novel poly(A)+ RNA-binding protein involved in the cytoplasmic delivery of messenger RNAs in yeast. Windgassen, M., Krebber, H. EMBO Rep. (2003) [Pubmed]
Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro. Lin, J.J., Zakian, V.A. Nucleic Acids Res. (1994) [Pubmed]
Exposure of single-stranded telomeric DNA causes G2/M cell cycle arrest in Saccharomyces cerevisiae. Pang, T.L., Wang, C.Y., Hsu, C.L., Chen, M.Y., Lin, J.J. J. Biol. Chem. (2003) [Pubmed]

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AGOS_AAL018W	Ashbya gossypii ATCC 10895
KLLA0D05016g	Kluyveromyces lactis NRRL Y-1140
HRB1	Saccharomyces cerevisiae S288c

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