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Gene Review:

RFX1 - Rfx1p

Saccharomyces cerevisiae S288c

Synonyms: CRT1, L9470.18, RFX-like DNA-binding protein RFX1, YLR176C

Huang, M. et al., Li, B. et al., Zaim, J. et al., Zhang, Z. et al., Klinkenberg, L.G. et al., et al.

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High impact information on RFX1

CRT1 encodes a DNA-binding protein that recruits the general repressors Ssn6 and Tup1 to the promoters of damage-inducible genes [1].
In response to DNA damage and replication blocks, Crt1 becomes hyperphosphorylated and no longer binds DNA, resulting in transcriptional induction [1].
The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor [1].
Molecular genetic analysis of the yeast repressor Rfx1/Crt1 reveals a novel two-step regulatory mechanism [2].
Here we have mapped the functional domains of Crt1 and identified two independent repression domains and a region required for gene activation [2].

Biological context of RFX1

Promoter mapping studies revealed that the upstream repressing sequences, the damage-responsive elements (DREs), rendered these genes dependent upon TAF(II)s. DREs are the binding sites for the sequence-specific DNA binding-protein Crt1 that represses transcription by recruiting the Ssn6-Tup1 co-repressor complex to the promoter [3].
We calculated the weight matrix representing the Crt1p binding site motif according to analysis of the 5'-UTR sequences of the genes that are under its regulation [4].
A statistical analysis showed that it is unlikely that a random gene cluster contains the motif conserved as well as the Crt1p binding site [4].

Associations of RFX1 with chemical compounds

We report here cross-regulation between Rox1 and Mot3 and Rfx1 in the regulation of the RNR genes encoding ribonucleotide diphosphate reductase [5].

Other interactions of RFX1

This contrasts with the derepression of Crt1-regulated genes, which requires both Rad53 and Dun1 protein kinases [6].

References

The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor. Huang, M., Zhou, Z., Elledge, S.J. Cell (1998) [Pubmed]
Molecular genetic analysis of the yeast repressor Rfx1/Crt1 reveals a novel two-step regulatory mechanism. Zhang, Z., Reese, J.C. Mol. Cell. Biol. (2005) [Pubmed]
Derepression of DNA damage-regulated genes requires yeast TAF(II)s. Li, B., Reese, J.C. EMBO J. (2000) [Pubmed]
Identification of new genes regulated by the Crt1 transcription factor, an effector of the DNA damage checkpoint pathway in Saccharomyces cerevisiae. Zaim, J., Speina, E., Kierzek, A.M. J. Biol. Chem. (2005) [Pubmed]
Synergy among differentially regulated repressors of the ribonucleotide diphosphate reductase genes of Saccharomyces cerevisiae. Klinkenberg, L.G., Webb, T., Zitomer, R.S. Eukaryotic Cell (2006) [Pubmed]
Phosphorylation of Rph1, a damage-responsive repressor of PHR1 in Saccharomyces cerevisiae, is dependent upon Rad53 kinase. Kim, E.M., Jang, Y.K., Park, S.D. Nucleic Acids Res. (2002) [Pubmed]

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