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Gene Review:

LYS20 - homocitrate synthase LYS20

Saccharomyces cerevisiae S288c

Synonyms: D1298, Homocitrate synthase, cytosolic isozyme, YDL182W

Ramos, F. et al., Chen, S. et al., Feller, A. et al., Gasent-Ramírez, J.M. et al., Verhasselt, P. et al., et al.

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Disease relevance of LYS20

During the sequencing of chromosome IV of S. cerevisiae, the sequence of ORF D1298 showing a significant similarity with the nifV gene of Azotobacter vinelandii was reported [1].

High impact information on LYS20

Immunoscreening a yeast expression library yielded five independent full-length clones of two open reading frames from chromosome IV, corresponding to YDL182w (LYS20) and YDL131w in the Saccharomyces genome data base [2].
Lysine overproduction by one of the mutants seemed to be due to, at least, the loss of repression of the homocitrate synthase encoded by the LYS20 gene [3].
In this report we demonstrate that Saccharomyces cerevisiae possesses two genes, LYS20 and LYS21, encoding two homocitrate synthase isoenzymes which are located in the nucleus [4].
The different isoforms are sensitive to inhibition by lysine but only the expression of LYS20 is strongly repressed by lysine [1].
The N-terminal end of homocitrate synthase isoform coded by LYS20 contains no typical mitochondrial targeting sequence, suggesting that this enzyme is not located in the mitochondria [1].

Chemical compound and disease context of LYS20

ORF D1298 shows the characteristics of alpha-isopropylmalate and homocitrate synthase genes and is similar to the nifV gene of Azotobacter vinelandii [5].

Biological context of LYS20

The Lys1p sequence shows 84% identity at the amino acid level with the putative product of open reading frame D1298 of S. cerevisiae [6].

Associations of LYS20 with chemical compounds

We have also shown that the product of LYS20 is responsible for the greater part of the lysine production [1].

References

Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae. Ramos, F., Verhasselt, P., Feller, A., Peeters, P., Wach, A., Dubois, E., Volckaert, G. Yeast (1996) [Pubmed]
Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae. Chen, S., Brockenbrough, J.S., Dove, J.E., Aris, J.P. J. Biol. Chem. (1997) [Pubmed]
Lysine-overproducing mutants of Saccharomyces cerevisiae baker's yeast isolated in continuous culture. Gasent-Ramírez, J.M., Benítez, T. Appl. Environ. Microbiol. (1997) [Pubmed]
In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Feller, A., Ramos, F., Piérard, A., Dubois, E. Eur. J. Biochem. (1999) [Pubmed]
New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae. Verhasselt, P., Voet, M., Volckaert, G. Yeast (1995) [Pubmed]
Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica. Perez-Campo, F.M., Nicaud, J.M., Gaillardin, C., Dominguez, A. Yeast (1996) [Pubmed]

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IMS2	Arabidopsis thaliana
MAM1	Arabidopsis thaliana
AGOS_ADR107W	Ashbya gossypii ATCC 10895
KLLA0F05489g	Kluyveromyces lactis NRRL Y-1140

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