High impact information on CDC36

• However, estrogen had no effect on late G1 arrest induced by the alpha factor or ts mutation of cdc36 [1].
• CDC4 and CDC36 are Saccharomyces cerevisiae cell division cycle genes, while ets is a transformation-specific sequence of avian erythroblastosis virus E26 [2].
• We report here significant primary sequence homology among the predicted translational products of three genes: CDC4 , CDC36 and ets [2].
• Allele-specific suppression, a two-hybrid interaction, and biochemical confractionation suggest that NOT1 and NOT2 are nuclear proteins associated in a discrete, 500-kD complex [3].
• This suggests that association of Not2 with the Ada/Gcn5 histone acetyltransferase complex is involved in regulation of the HIS3 promoter [4].

Biological context of CDC36

• These phenotypes are similar to those associated with temperature-sensitive mutations in CDC36 and CDC39, which are proposed to encode general negative regulators of transcription rather than factors involved in the pheromone response pathway [5].
• CDC36 and CDC39 are negative elements in the signal transduction pathway of yeast [6].
• Mutations in either the CDC36 or CDC39 gene cause yeast cells to arrest in G1 of the cell cycle at the same point as treatment with mating pheromone [6].
• The nucleotide sequences of the yeast cell division cycle start genes CDC36, CDC37, and CDC39 are presented [7].
• Our observations are consistent with the hypothesis that CDC28, CDC36 and CDC39 are required for irreversible commitment to nuclear division in both the mitotic and meiotic pathways [8].

Physical interactions of CDC36

• The not2::R165G also abrogated NOT2 ability to interact with ADA2 but had little effect on the integrity of the CCR4-NOT complex [9].

Regulatory relationships of CDC36

• Overexpression of MOT2 suppresses the growth defect of temperature-sensitive mutations in CDC36 and CDC39 [5].

Other interactions of CDC36

• The coding regions corresponding to CDC36, CDC37, and CDC39 were then identified and localized by R-loop analysis [10].
• The CDC9 gene of Saccharomyces cerevisiae encodes a DNA ligase, and we have determined the nucleotide sequence of a 3.85 kb fragment of DNA which encompasses the convergently transcribed CDC9 and CDC36 genes [11].
• Alterations in NOT2 contacts to ADA2, therefore, do not necessarily result in effects on the CCR4-NOT complex nor result in severe growth defects [9].
• In contrast, the not2-4 allele destabilized the CCR4-NOT complex to a lesser extent and had no effect on NOT5 association with NOT2 [9].
• Alpha-factor-treated cells arrested in a thermosensitive state, as did the temperature-sensitive mutant cdc36 when grown at the restrictive temperature. cdc7, which arrested at the G1-S boundary, arrested in a thermosensitive state [12].

References