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MOT2  -  CCR4-NOT core ubiquitin-protein ligase...

Saccharomyces cerevisiae S288c

Synonyms: CCL1, General negative regulator of transcription subunit 4, Modulator of transcription 2, NOT4, SIG1, ...
 
 
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Disease relevance of MOT2

  • On the other hand, sterility resulting from deletion of STE4 was not suppressed by the mot2 deletion [1].
 

High impact information on MOT2

  • Four genes, NOT1(CDC39), NOT2(CDC36), NOT3, NOT4, act as general negative regulators and preferentially affect TC-dependent transcription [2].
  • In this paper, we show interactions between the essential domain of Not1p, which interacts with Not4p and Not5p, and the N-terminal domain of yTAF1 [3].
  • We isolated a temperature-sensitive nonsense allele of TAF1, taf1-4, which is synthetically lethal at the permissive temperature when combined with not4 and not5 mutants and which produces high levels of a C-terminally truncated yTAF1 derivative [3].
  • Overexpression of C-terminally truncated yTAF1 is toxic in not4 or not5 mutants, whereas overexpression of full-length yTAF1 suppresses not4 [3].
  • The MOT2 gene encodes a putative zinc finger protein, the deletion of which resulted in temperature-sensitive growth, increased expression of FUS1 in the absence of pheromones, and suppression of a deletion of the alpha-factor receptor [1].
 

Biological context of MOT2

  • The yeast MOT2 gene encodes a putative zinc finger protein that serves as a global negative regulator affecting expression of several categories of genes, including mating-pheromone-responsive genes [1].
  • MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae [4].
  • The suppressor mot2 was isolated as a recessive mutation that restored conjugational competence to a temperature-sensitive ste4 mutant and simultaneously conferred a temperature-sensitive growth phenotype [1].
  • Deletion of the NOT4 gene impairs hyphal development and pathogenicity in Candida albicans [5].
  • These findings suggest that NOT4 may play a role in affecting strain pathogenicity, possibly by regulating expression of certain genes that effect cellular morphogenesis and virulence [5].
 

Associations of MOT2 with chemical compounds

  • First, defects in CCR4-NOT components as well as overexpression of the NOT4 gene elicited 6-azauracil (6AU) and mycophenolic acid sensitivities, hallmarks of transcriptional elongation defects [6].
  • K. lactis strains lacking Not4p are defective in fermentation and show reduced transcription of glucose transporter and glycolytic genes, which are phenotypes that are not found in the corresponding mutant of S. cerevisiae [7].
  • We found that the ubiquitin protein ligase activity of Not4p does not play a role in HU induced Sml1p degradation [8].
 

Regulatory relationships of MOT2

 

Other interactions of MOT2

  • Secondly, mutations in NOT4 are synthetically lethal with mutations in NOT5 [9].
  • These observations suggest that Mot2 functions as a general negative regulator of transcription in the same processes as Cdc36 and Cdc39 [1].
  • We isolated conditional taf19 alleles that display synthetic growth phenotypes when combined with not4 or specific not5 alleles [10].
  • In the absence of Not4p, Egd2p mislocalizes to punctuate structures [11].
  • Ubiquitination of Egd1p requires Not4p [11].
 

Analytical, diagnostic and therapeutic context of MOT2

References

  1. The yeast MOT2 gene encodes a putative zinc finger protein that serves as a global negative regulator affecting expression of several categories of genes, including mating-pheromone-responsive genes. Irie, K., Yamaguchi, K., Kawase, K., Matsumoto, K. Mol. Cell. Biol. (1994) [Pubmed]
  2. NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. Collart, M.A., Struhl, K. Genes Dev. (1994) [Pubmed]
  3. The Ccr4-not complex and yTAF1 (yTaf(II)130p/yTaf(II)145p) show physical and functional interactions. Deluen, C., James, N., Maillet, L., Molinete, M., Theiler, G., Lemaire, M., Paquet, N., Collart, M.A. Mol. Cell. Biol. (2002) [Pubmed]
  4. MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae. Cade, R.M., Errede, B. Mol. Cell. Biol. (1994) [Pubmed]
  5. Deletion of the NOT4 gene impairs hyphal development and pathogenicity in Candida albicans. Krueger, K.E., Ghosh, A.K., Krom, B.P., Cihlar, R.L. Microbiology (Reading, Engl.) (2004) [Pubmed]
  6. Genetic evidence supports a role for the yeast CCR4-NOT complex in transcriptional elongation. Denis, C.L., Chiang, Y.C., Cui, Y., Chen, J. Genetics (2001) [Pubmed]
  7. The inactivation of KlNOT4, a Kluyveromyces lactis gene encoding a component of the CCR4-NOT complex, reveals new regulatory functions. Mazzoni, C., Serafini, A., Falcone, C. Genetics (2005) [Pubmed]
  8. DNA damage and replication stress induced transcription of RNR genes is dependent on the Ccr4-Not complex. Mulder, K.W., Winkler, G.S., Timmers, H.T. Nucleic Acids Res. (2005) [Pubmed]
  9. Characterization of NOT5 that encodes a new component of the Not protein complex. Oberholzer, U., Collart, M.A. Gene (1998) [Pubmed]
  10. The TATA-binding protein-associated factor yTafII19p functionally interacts with components of the global transcriptional regulator Ccr4-Not complex and physically interacts with the Not5 subunit. Lemaire, M., Collart, M.A. J. Biol. Chem. (2000) [Pubmed]
  11. The Yeast Ccr4-Not Complex Controls Ubiquitination of the Nascent-associated Polypeptide (NAC-EGD) Complex. Panasenko, O., Landrieux, E., Feuermann, M., Finka, A., Paquet, N., Collart, M.A. J. Biol. Chem. (2006) [Pubmed]
 
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