High impact information on TOM6

• Tom40 is an essential constituent of the GIP, whereas Tom6 and Tom7 modulate the assembly and dissociation of the Tom machinery [1].
• Immunoprecipitation of the gene product, ISP42p, from mitochondria solubilized under mild conditions reveals a multi-protein complex containing ISP6p and ISP42p [2].
• Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex [2].
• This novel gene, denoted ISP6, encodes a 61 amino acid integral membrane protein of the mitochondrial outer membrane, which is oriented with its amino-terminus facing the cytosol [2].
• Tom7 and Tom6 have been found in Neurospora and yeast, Tom5 has been found so far only in the latter organism [3].

Biological context of TOM6

• Biogenesis of ISP6, a small carboxyl-terminal anchored protein of the receptor complex of the mitochondrial outer membrane [4].
• The insertion pathway of these proteins shares a common binding site with the general import pathway as the assembly of both Tom6 and Tom7 was competed by a matrix-destined precursor protein [5].
• In vivo this fusion protein lost its ability to suppress the temperature-sensitive phenotype of an isp42 mutant, while the other fusion protein DHFR-6, which was found targeted correctly to mitochondria, suppressed the mutant as well as the wild-type ISP6 [4].

Physical interactions of TOM6

• Tom40 exists in a homo-oligomeric assembly and dynamically interacts with Tom6 [6].
• In addition, Tom6 was observed to interact with Tom22 in a manner that depends on the presence of preproteins in transit [5].

Other interactions of TOM6

• Disruption of the ISP6 gene is without apparent effect in wild type yeast cells, but is lethal in temperature-sensitive isp42 mutants [2].

References