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Gene Review

HAP5  -  Hap5p

Saccharomyces cerevisiae S288c

Synonyms: Transcriptional activator HAP5, YOR358W
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High impact information on HAP5

  • Furthermore, we demonstrate that purified recombinant HAP2, HAP3, and HAP5 are able to reconstitute CCAAT-binding activity in mobility shift analysis [1].
  • We now report the isolation of the genes hapB and hapE, which encode proteins with central regions of high similarity to Hap2p and Hap5p of S. cerevisiae and to the CBF-B and CBF-C proteins of mammals [2].
  • A second domain that is essential for the recruitment of Hap4p into the CCAAT-binding complex was identified in Hap5p and Php5p [3].
  • Analysis of the evolutionarily conserved regions of Hap5p, Php5p, and the mammalian homolog CBF-C revealed two essential domains within Hap5p that are required for DNA binding and transcriptional activation [3].
  • Chromatin immunoprecipitation demonstrated that TCOL is recruited to the CCAAT motifs of the yeast CYC1 and HEM1 promoters by HAP5 [4].

Biological context of HAP5


Associations of HAP5 with chemical compounds


Physical interactions of HAP5

  • The Hap2p/Hap3p/Hap5p heterotrimer is the DNA-binding component of the complex that binds to the consensus 5'-CCAAT-3' sequence in the promoter of target genes [7].

Other interactions of HAP5

  • RT-PCR analyses revealed that the expression of SIP4 and HAP5, which are known to affect the expression of some of the gluconeogenic, TCA cycle and respiratory genes, were also increased under this condition [8].


  1. Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding. McNabb, D.S., Xing, Y., Guarente, L. Genes Dev. (1995) [Pubmed]
  2. AnCF, the CCAAT binding complex of Aspergillus nidulans, contains products of the hapB, hapC, and hapE genes and is required for activation by the pathway-specific regulatory gene amdR. Steidl, S., Papagiannopoulos, P., Litzka, O., Andrianopoulos, A., Davis, M.A., Brakhage, A.A., Hynes, M.J. Mol. Cell. Biol. (1999) [Pubmed]
  3. The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor. McNabb, D.S., Tseng, K.A., Guarente, L. Mol. Cell. Biol. (1997) [Pubmed]
  4. The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA. Ben-Naim, O., Eshed, R., Parnis, A., Teper-Bamnolker, P., Shalit, A., Coupland, G., Samach, A., Lifschitz, E. Plant J. (2006) [Pubmed]
  5. The Neurospora aab-1 gene encodes a CCAAT binding protein homologous to yeast HAP5. Chen, H., Crabb, J.W., Kinsey, J.A. Genetics (1998) [Pubmed]
  6. The Region in a Subunit of the Aspergillus CCAAT-Binding Protein Similar to the HAP4p-Recruiting Domain of Saccharomyces cerevisiae Hap5p Is Not Essential for Transcriptional Enhancement. Tanoue, S., Kamei, K., Goda, H., Tanaka, A., Kobayashi, T., Tsukagoshi, N., Kato, M. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
  7. Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae. McNabb, D.S., Pinto, I. Eukaryotic Cell (2005) [Pubmed]
  8. Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae. Sasaki, H., Uemura, H. Yeast (2005) [Pubmed]
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