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Gene Review

SIP4  -  Sip4p

Saccharomyces cerevisiae S288c

Synonyms: J0922, Protein SIP4, YJL089W
 
 
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Disease relevance of SIP4

 

High impact information on SIP4

  • The sip1Delta sip2Delta gal83Delta strain is unable to derepress invertase, grows poorly on alternative carbon sources and fails to direct the phosphorylation of the Mig1 and Sip4 proteins in vivo [2].
  • Indeed, deletion of individual beta-subunit genes causes distinct differences in the induction and phosphorylation of Sip4, strongly suggesting that the beta-subunits play an important role in substrate definition [2].
  • Gal83 mediates the interaction of the Snf1 kinase complex with the transcription activator Sip4 [3].
  • Gal83 interacts with Sip4 in two-hybrid assays in vivo, and bacterially expressed proteins bind in vitro [3].
  • We present genetic evidence that Sip4 contributes to transcriptional activation by the CSRE and biochemical evidence that Sip4 binds to the CSRE [4].
 

Biological context of SIP4

  • The positively acting regulatory genes CAT8 and SIP4 encode CSRE-binding proteins which contribute unequally to the regulated expression of a CSRE-dependent reporter gene (85% and 15%, respectively, under conditions of glucose derepression) [5].
  • This finding agrees with the idea that phosphorylation by Cat1 may convert Sip4 into a functional activator [5].
  • Contribution of Cat8 and Sip4 to the transcriptional activation of yeast gluconeogenic genes by carbon source-responsive elements [5].
  • Deregulated variants of Cat8 and Sip4 are able to bind to the CSRE and allow glucose-insensitive gene activation, even in the absence of the other protein, arguing against the physiological significance of heterodimer formation [5].
  • In a diploid disruption mutant of ORF J0922 coding for the transcriptional activator homologue, no colonies appeared before 10 days after transformation and then grew slowly [6].
 

Associations of SIP4 with chemical compounds

  • Regulatory genes CAT8 and SIP4 both encode zinc-cluster proteins which can bind to CSRE motifs and activate target genes under conditions of glucose deprivation [7].
  • The N terminus of the predicted 96-kDa SIP4 protein is homologous to the DNA-binding domain of the GAL4 family of transcriptional activators, with a C6 zinc cluster adjacent to a coiled-coil motif The C terminus contains a leucine zipper motif and an acidic region [8].
 

Regulatory relationships of SIP4

  • The derepression deficiency of a CSRE-dependent reporter gene in a strain lacking the Cat1 (Snf1) protein kinase can be suppressed by Sip4 fused to a strong heterologous activation domain [5].
 

Other interactions of SIP4

  • RT-PCR analyses revealed that the expression of SIP4 and HAP5, which are known to affect the expression of some of the gluconeogenic, TCA cycle and respiratory genes, were also increased under this condition [9].
  • We show that Gal83 mediates the association of the kinase with Sip4, a Snf1-regulated transcription activator of gluconeogenic genes [3].
  • Interaction of the Srb10 kinase with Sip4, a transcriptional activator of gluconeogenic genes in Saccharomyces cerevisiae [10].
 

Analytical, diagnostic and therapeutic context of SIP4

References

  1. Cat8 and Sip4 mediate regulated transcriptional activation of the yeast malate dehydrogenase gene MDH2 by three carbon source-responsive promoter elements. Roth, S., Schüller, H.J. Yeast (2001) [Pubmed]
  2. beta-subunits of Snf1 kinase are required for kinase function and substrate definition. Schmidt, M.C., McCartney, R.R. EMBO J. (2000) [Pubmed]
  3. Gal83 mediates the interaction of the Snf1 kinase complex with the transcription activator Sip4. Vincent, O., Carlson, M. EMBO J. (1999) [Pubmed]
  4. Sip4, a Snf1 kinase-dependent transcriptional activator, binds to the carbon source-responsive element of gluconeogenic genes. Vincent, O., Carlson, M. EMBO J. (1998) [Pubmed]
  5. Contribution of Cat8 and Sip4 to the transcriptional activation of yeast gluconeogenic genes by carbon source-responsive elements. Hiesinger, M., Roth, S., Meissner, E., Schüller, H.J. Curr. Genet. (2001) [Pubmed]
  6. Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site. Miosga, T., Schaaff-Gerstenschläger, I., Chalwatzis, N., Baur, A., Boles, E., Fournier, C., Schmitt, S., Velten, C., Wilhelm, N., Zimmermann, F.K. Yeast (1995) [Pubmed]
  7. Transcriptional activators Cat8 and Sip4 discriminate between sequence variants of the carbon source-responsive promoter element in the yeast Saccharomyces cerevisiae. Roth, S., Kumme, J., Schüller, H.J. Curr. Genet. (2004) [Pubmed]
  8. Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response. Lesage, P., Yang, X., Carlson, M. Mol. Cell. Biol. (1996) [Pubmed]
  9. Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae. Sasaki, H., Uemura, H. Yeast (2005) [Pubmed]
  10. Interaction of the Srb10 kinase with Sip4, a transcriptional activator of gluconeogenic genes in Saccharomyces cerevisiae. Vincent, O., Kuchin, S., Hong, S.P., Townley, R., Vyas, V.K., Carlson, M. Mol. Cell. Biol. (2001) [Pubmed]
 
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