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Gene Review:

SCEI - intron-encoded endonuclease I-SceI

Saccharomyces cerevisiae S288c

Synonyms: 21S rRNA intron maturase, Homing endonuclease omega, Intron-encoded endonuclease I-SceI, OMEGA, Q0160, ...

Wells, D.R. et al., Fetrow, J.S. et al., Zhu, H. et al., Choulika, A. et al., Wang, X. et al., et al.

Fetrow, Spitzer, Gilden, Mellender, Begley, Haas, Boose,

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Disease relevance of SCEI

A supportive correlation is that Omega and Cjw1, which are distinguished from other mycobacteriophages by their possession of a Pnkp enzyme, are also unique among the mycobacteriophages in their specification of putative RNA ligases [1].

High impact information on SCEI

A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation [2].
Sequence analysis of a 102-kD protein, identified previously as a specific Omega RNA-binding protein, revealed homology to the HSP101/HSP104/ClpB family of heat shock proteins and its expression in yeast complemented a thermotolerance defect caused by a deletion of the HSP104 gene [3].
The 5' leader (Omega) of tobacco mosaic viral RNA functions as a translational enhancer [3].
The mitochondrial intron-encoded endonuclease I-SceI of Saccharomyces cerevisiae has an 18-bp recognition sequence and, therefore, has a very low probability of cutting DNA, even within large genomes [4].
Residues 75-78 form a tight turn within Omega-loop D in Saccharomyces cerevisiae iso-1-cytochrome c. Directed, random mutagenesis of invariant residues proline 76 and glycine 77 in this turn were analyzed for the in vivo functionality and level of protein within the cell [5].

Associations of SCEI with chemical compounds

Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases [6].

Analytical, diagnostic and therapeutic context of SCEI

The optimized electroporation condition was 900 V, 25 microF, and 200 Omega. The DNA concentration did not influence the transformation [7].

References

Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40. Zhu, H., Yin, S., Shuman, S. J. Biol. Chem. (2004) [Pubmed]
A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation. Görlich, D., Prehn, S., Hartmann, E., Kalies, K.U., Rapoport, T.A. Cell (1992) [Pubmed]
HSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status. Wells, D.R., Tanguay, R.L., Le, H., Gallie, D.R. Genes Dev. (1998) [Pubmed]
Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae. Choulika, A., Perrin, A., Dujon, B., Nicolas, J.F. Mol. Cell. Biol. (1995) [Pubmed]
Structure, function, and temperature sensitivity of directed, random mutants at proline 76 and glycine 77 in omega-loop D of yeast iso-1-cytochrome c. Fetrow, J.S., Spitzer, J.S., Gilden, B.M., Mellender, S.J., Begley, T.J., Haas, B.J., Boose, T.L. Biochemistry (1998) [Pubmed]
Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases. Garcerá, A., Barreto, L., Piedrafita, L., Tamarit, J., Herrero, E. Biochem. J. (2006) [Pubmed]
A site-directed integration system for the nonuniversal CUGSer codon usage species Pichia farinosa by electroporation. Wang, X., Li, G., Deng, Y., Yu, X., Chen, F. Arch. Microbiol. (2006) [Pubmed]

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