Disease relevance of NCE103

• Consistent with this conclusion, recombinant NCE103 synthesized in Escherichia coli shows CA activity, and Delta NCE103 expressing the tobacco chloroplast CA exhibits the same sensitivity to H2O2 as the wild-type strain [1].

High impact information on NCE103

• Northern blot analysis revealed that that expression of NCE103 is transcriptionally regulated by low Ci which was also demonstrated by fusing the NCE103 promoter to beta-galactosidase as a reporter gene [2].
• No carbonic anhydrase activity could be detected in crude extracts prepared from wild-type, nce103-Delta mutants or in strains transformed with a multicopy plasmid carrying the NCE103 gene [3].
• However, the predicted amino acid sequence of Nce103p shows high levels of identities to carbonic anhydrases of pro- and eukaryotes [3].
• Other transcripts that showed a significant transcriptional response to elevated CO(2) included NCE103 (probably encoding carbonic anhydrase), PCK1 (encoding PEP carboxykinase) and members of the IMD gene family (encoding isozymes of inosine monophosphate dehydrogenase) [4].
• To discriminate between 'sparking' effects of CO2 and a CO2 requirement for steady-state fermentative growth, we switched glucose-limited anaerobic chemostat cultures of an nce103 null mutant from sparging with pure CO2 to sparging with nitrogen gas [5].

Associations of NCE103 with chemical compounds

• Nutritional analysis of the nce103 null mutant demonstrated that growth on glucose under a non-CO2-enriched nitrogen atmosphere was possible when the culture medium was provided with L-aspartate, fatty acids, uracil and L-argininine [5].

References