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Gene Review

PCK1  -  phosphoenolpyruvate carboxykinase PCK1

Saccharomyces cerevisiae S288c

Synonyms: JPM2, PEPC, PPC1, YKR097W
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Disease relevance of PCK1


High impact information on PCK1

  • PCK1 encoding phosphoenolpyruvate carboxykinase is transcriptionally regulated by two upstream activating elements [3].
  • To study the phenotype of C. albicans diploid cells depleted of CaCse4p, we deleted one copy of CaCSE4 and brought the other copy under control of a regulated PCK1 promoter (repressed by glucose and induced by succinate) [4].
  • The occurrence of Cat8pIII was strongly correlated with the derepression of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase and fructose-1,6-bisphosphatase) and gluconeogenic PCK1 mRNA [5].
  • This phenotypic defect is similar to that associated with deletion of the BCK1 gene, which is thought to function in the pathway mediated by PCK1 [6].
  • The data show that yeast cells can detect very low glucose concentrations in the environment, and suggest that several distinct signalling pathways operate to repress FPB1 and PCK1 transcription in the presence of glucose [7].

Biological context of PCK1


Associations of PCK1 with chemical compounds

  • Similarly, of 6144 ORFs, 72 (1.17%) showed greater than a 1.4-fold decrease in transcript level and only one of these, PCK1, was decreased greater than two-fold Functional categories of genes that were induced by sorbic acid stress included cell stress (particularly oxidative stress), transposon function, mating response and energy generation [12].
  • Other transcripts that showed a significant transcriptional response to elevated CO(2) included NCE103 (probably encoding carbonic anhydrase), PCK1 (encoding PEP carboxykinase) and members of the IMD gene family (encoding isozymes of inosine monophosphate dehydrogenase) [13].
  • Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae [14].
  • This up/down-regulation of PEPC is catalyzed by a dedicated and highly regulated serine/threonine (Ser/Thr) kinase (PEPC-kinase) and an opposing type-2A Ser/Thr phosphatase (PP2A) [15].
  • This report represents one of the few studies of a heteromeric PP2A holoenzyme from photosynthetic tissue that dephosphorylates a known target enzyme in plants, such as PEPC, sucrose-phosphate synthase or nitrate reductase [15].

Physical interactions of PCK1

  • Consistent with this, synthetic genetic interactions were observed between the genes encoding the Cdc34/SCF complex and key components of the Pck1-Slt2 MAPK pathway [16].

Other interactions of PCK1


Analytical, diagnostic and therapeutic context of PCK1


  1. Cloning and characterisation of pepC, a gene encoding a serine protease from Aspergillus niger. Frederick, G.D., Rombouts, P., Buxton, F.P. Gene (1993) [Pubmed]
  2. Divergence of eukaryotic secretory components: the Candida albicans homolog of the Saccharomyces cerevisiae ++Sec20 protein is N terminally truncated, and its levels determine antifungal drug resistance and growth. Weber, Y., Santore, U.J., Ernst, J.F., Swoboda, R.K. J. Bacteriol. (2001) [Pubmed]
  3. CAT5, a new gene necessary for derepression of gluconeogenic enzymes in Saccharomyces cerevisiae. Proft, M., Kötter, P., Hedges, D., Bojunga, N., Entian, K.D. EMBO J. (1995) [Pubmed]
  4. The CENP-A homolog CaCse4p in the pathogenic yeast Candida albicans is a centromere protein essential for chromosome transmission. Sanyal, K., Carbon, J. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  5. Glucose derepression of gluconeogenic enzymes in Saccharomyces cerevisiae correlates with phosphorylation of the gene activator Cat8p. Randez-Gil, F., Bojunga, N., Proft, M., Entian, K.D. Mol. Cell. Biol. (1997) [Pubmed]
  6. MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C. Irie, K., Takase, M., Lee, K.S., Levin, D.E., Araki, H., Matsumoto, K., Oshima, Y. Mol. Cell. Biol. (1993) [Pubmed]
  7. Multiple signalling pathways trigger the exquisite sensitivity of yeast gluconeogenic mRNAs to glucose. Yin, Z., Smith, R.J., Brown, A.J. Mol. Microbiol. (1996) [Pubmed]
  8. Regulatory regions in the yeast FBP1 and PCK1 genes. Mercado, J.J., Gancedo, J.M. FEBS Lett. (1992) [Pubmed]
  9. Differences in regulation of yeast gluconeogenesis revealed by Cat8p-independent activation of PCK1 and FBP1 genes in Kluyveromyces lactis. Georis, I., Krijger, J.J., Breunig, K.D., Vandenhaute, J. Mol. Gen. Genet. (2000) [Pubmed]
  10. Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae. Valdés-Hevia, M.D., de la Guerra, R., Gancedo, C. FEBS Lett. (1989) [Pubmed]
  11. Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans. Leuker, C.E., Sonneborn, A., Delbrück, S., Ernst, J.F. Gene (1997) [Pubmed]
  12. Parallel and comparative analysis of the proteome and transcriptome of sorbic acid-stressed Saccharomyces cerevisiae. de Nobel, H., Lawrie, L., Brul, S., Klis, F., Davis, M., Alloush, H., Coote, P. Yeast (2001) [Pubmed]
  13. Physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to high carbon dioxide concentrations. Aguilera, J., Petit, T., de Winde, J.H., Pronk, J.T. FEMS Yeast Res. (2005) [Pubmed]
  14. Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae. Casal, M., Blázquez, M.A., Gamo, F.J., Gancedo, C., Leão, C. FEMS Microbiol. Lett. (1995) [Pubmed]
  15. Partial purification and biochemical characterization of a heteromeric protein phosphatase 2A holoenzyme from maize (Zea mays L.) leaves that dephosphorylates C4 phosophoenolpyruvate carboxylase. Dong, L., Ermolova, N.V., Chollet, R. Planta (2001) [Pubmed]
  16. The Cdc34/SCF Ubiquitination Complex Mediates Saccharomyces cerevisiae Cell Wall Integrity. Varelas, X., Stuart, D., Ellison, M.J., Ptak, C. Genetics (2006) [Pubmed]
  17. Mapping of the PCK1 gene encoding phosphoenolpyruvate carboxykinase on chromosome XI of Saccharomyces cerevisiae. Delgado, M.A., Gancedo, C. FEMS Microbiol. Lett. (1992) [Pubmed]
  18. Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis. Kitamoto, H.K., Ohmomo, S., Iimura, Y. Yeast (1998) [Pubmed]
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