High impact information on SUT2

• Sequencing of the genes revealed open reading frames coding for 553 amino acids in the case of SUT1, and for 550 amino acids in the case of SUT2 and of SUT3 [1].
• SUT2 was found in a screen for multicopy suppressors of the synthetic slow growth phenotype of a Deltaras2Deltagpa2 double deletion mutant [2].
• Additionally, we demonstrate that cellular levels of Ras2p are affected by Sut2p and that Sut2-GFPp accumulates significantly in the nucleus [2].
• By testing cAMP-dependent reactions including the accumulation of storage carbohydrates, pseudohyphal differentiation, entry of meiosis as well as the measurement of FLO11 reporter activity we show that Sut2p modulates the activity of protein kinase A (PKA) [2].

Biological context of SUT2

• Based on the observed influence of high SUT2 gene dosage on PKA activity as well as Sut2p's homology to the presumptive transcription factor Sut1p, we suggest that Sut2p contributes to regulation of PKA activity at the level of transcription [2].
• Determination of substrate specificities and kinetic parameters of the individual Sut proteins expressed in a S. cerevisiae hxt1-7 mutant revealed Sut1, Sut2 and Sut3 as glucose transporters with K(m) values in the millimolar range [1].

Associations of SUT2 with chemical compounds

• We have identified and characterized three genes, SUT1, SUT2 and SUT3, that encode glucose transporters of the yeast Pichia stipitis [1].
• Since the N terminus of SUT2 determines affinity for sucrose, the reconstituted chimera has lower affinity than SUT1 [3].

Physical interactions of SUT2

• Furthermore, the N-terminal half of the low-affinity SUT2 interacts functionally with the C-terminal half of SUT1 [3].

Other interactions of SUT2

• Moreover, here we present new data showing that the structurally related YPR009 gene (SUT2) is a functional homologue of SUT1, and that both gene products may represent two novel yeast regulatory proteins involved in sterol uptake [4].

References