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GDB1  -  bifunctional 4-alpha-glucanotransferase/am...

Saccharomyces cerevisiae S288c

Synonyms: Glycogen debrancher, Glycogen debranching enzyme, YPR184W
 
 
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Disease relevance of GDB1

 

High impact information on GDB1

  • These results indicated that GDE catalyzed cyclization of amylose to produce cyclic alpha-1,4 glucan (cycloamylose) [2].
  • GDE also efficiently produced cycloamylose even from the branched glucan substrate, starch, demonstrating its potential for industrial production of cycloamylose [2].
  • GDE treatment resulted in a rapid reduction of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exo-amylase (glucoamylase [GA]) but was degraded by an endo-type alpha-amylase to glucose and maltose [2].
  • A 99.3% homology was found between the nucleotide sequences of GDE gene harbored in the recombinant E. coli plasmid (pTrc99A) and the open reading frame (902039-906646 position) of the 4608-bp fragment of S. cerevisiae chromosome XVI [1].
  • When the cultivation temperature of recombinant E. coli strains was lowered to 25 degrees C and the isopropyl-beta-d-thiogalactopyranoside (IPTG) concentration used for induction was decreased to as low as 0.02 mM, a total of about 33 mg of recombinant GDE can be isolated from a liter culture as estimated by amylo-1,6-glucosidase activity [1].
 

Biological context of GDB1

References

  1. High expression of glycogen-debranching enzyme in Escherichia coli and its competent purification method. Nakayama, A., Yamamoto, K., Tabata, S. Protein Expr. Purif. (2000) [Pubmed]
  2. Cyclization reaction catalyzed by glycogen debranching enzyme (EC 2.4.1.25/EC 3.2.1.33) and its potential for cycloamylose production. Yanase, M., Takata, H., Takaha, T., Kuriki, T., Smith, S.M., Okada, S. Appl. Environ. Microbiol. (2002) [Pubmed]
 
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