High impact information on Nnat

• Therefore, we studied the expression of neuronatin in PC12 cells, an established model of neuronal growth and differentiation [1].
• Furthermore, on sequencing the human neuronatin gene, it was determined that the alpha-form was encoded by three exons, and the beta-form was encoded by the first and third exons only (Genomics, 33 (1996) 292-297) [1].
• Further studies described in the present manuscript, lead to the identification of two alternatively spliced forms of neuronatin mRNA, alpha and beta, with the same open reading frame [2].
• Neuronatin mRNA: alternatively spliced forms of a novel brain-specific mammalian developmental gene [2].
• The selective expression of this novel mRNA in late fetal and early postnatal brain development, and loss of expression in adulthood and senescence, suggests that downregulation of neuronatin may be involved in terminal brain differentiation [3].

Associations of Nnat with chemical compounds

• After the neuronatin-beta gene was transfected into and expressed in the 1.9 cells, they regained wild type PC12 levels of resistance to nigericin, rotenone and valinomycin [4].
• We have isolated a PC12 variant, called 1.9, that retains many of the neuron-like properties of wild type PC12 cells, but it does not express neuronatin and it exhibits markedly increased sensitivity to the toxic effects of nigericin, rotenone and valinomycin [4].

Analytical, diagnostic and therapeutic context of Nnat

• The RT-PCR results clearly indicated that there were only two forms of neuronatin [2].
• Molecular cloning of a novel mRNA (neuronatin) that is highly expressed in neonatal mammalian brain [3].

References