Disease relevance of murD

• Nucleotide sequence involving murD and an open reading frame ORF-Y spacing murF and ftsW in Escherichia coli [1].
• The murD gene was cloned from both Staphylococcus aureus and Streptococcus pyogenes [2].
• DNA sequence of the murE-murD region of Bacillus subtilis 168 [3].

High impact information on murD

• In the absence of exogenous promoters, plasmid-mediated complementation of the temperature-sensitive ftsW201 allele requires the presence of the full coding sequence of ftsW plus upstream DNA encompassing the C-terminus of mraY and the full coding sequence of murD [4].
• Nucleotide sequence of the murD gene encoding the UDP-MurNAc-L-Ala-D-Glu synthetase of Escherichia coli [5].
• The genes (murD) that encode these enzymes were cloned into pMAL-c2 fusion vector and overexpressed as maltose-binding protein-MurD fusion proteins [6].
• Sequence analysis of the S. aureus murD gene revealed an open reading frame of 449 amino acids [2].
• Cloning and expression of Staphylococcus aureus and Treptococcus pyogenes murD genes encoding uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligases [2].

Biological context of murD

• Although the Rm1021 fragment shares no significant nucleotide sequence identity with Ec16 pelB, it includes an ORF (open reading frame) that shares a high degree of nt sequence identity with the Escherichia coli murD gene [7].
• The UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase of Escherichia coli was over-produced in strains that harbour recombinant plasmids bearing the murD gene under the control of the lac or PR promoter [8].

Anatomical context of murD

• This gene codes for UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase, which catalyzes a step in the synthesis of the E. coli cell wall [7].

Other interactions of murD

• During vegetative growth and early stationary phase spoVE is cotranscribed with murD and murG in the form of very long polycistronic messages originating upstream of murD [9].

References