Disease relevance of murF

• Contribution of the Pmra promoter to expression of genes in the Escherichia coli mra cluster of cell envelope biosynthesis and cell division genes [1].
• We have cloned the Pseudomonas aeruginosa cell wall biosynthesis and cell division gene cluster that corresponds to the mra operon in the 2-min region of the Escherichia coli chromosome [2].
• Characterization of the murF gene of the cyanobacterium Synechocystis sp. PCC 6803 [3].

High impact information on murF

• Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli [4].
• Nucleotide sequence involving murD and an open reading frame ORF-Y spacing murF and ftsW in Escherichia coli [5].
• Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli [6].
• Two highly conserved residues in region II were examined through site-directed mutagenesis of the murein D-alanyl-D-alanine-adding enzyme from Escherichia coli (murF; E158 and H188) [7].
• Disruption of this promoter, named the mra promoter, on the chromosome by inserting the lac promoter led to cell lysis in the absence of a lac inducer [8].

Biological context of murF

• The murF gene is present as a single-copy gene in the genome of Synechocystis sp. PCC 6803 [3].
• The amino acid sequence deduced from the murF gene is 39% identical to that of the product of the murF gene of Escherichia coli [3].
• Examination of the controls exerted on the mra operon shows that E. coli uses an extraordinary range of strategies to co-ordinate the expression of the cell division genes with respect to each other and to the cell cycle [9].

Associations of murF with chemical compounds

• The D-alanyl-D-alanine-adding enzyme encoded by the murF gene catalyzes the ATP-dependent formation of UDP-N-acetylmuramyl-L-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala (UDP-MurNAc-tripeptide) [10].
• The Actinoplanes teichomyceticus tcp cluster, devoted to the biosynthesis of the glycopeptide antibiotic teicoplanin, contains van genes associated to a murF-like sequence (murF2) [11].

Other interactions of murF

• Determination of gene products and coding regions from the murE-murF region of Escherichia coli [12].
• Three new mutants of Escherichia coli showing thermosensitive cell growth and division were isolated, and the mutations were mapped to the mra region at 2 min on the E. coli chromosome map distal to leuA [13].

References