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Gene Review

gshA  -  glutamate-cysteine ligase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2683, JW2663, gshI
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Disease relevance of gshA

  • METHODS AND RESULTS: GSH production was very low in E. coli by the expression of gshI gene [1].

High impact information on gshA

  • A knockout of gshA, involved in glutathione biosynthesis, enhanced the susceptibility to metronidazole, while a knockout of recD, involved in recombination and repair, made the bacteria hypersusceptible to ciprofloxacin [2].
  • To clone gshI, chromosomal DNAs of RC912 and plasmid vector pBR322 were digested with various restriction endonucleases and then ligated with T4 DNA ligase [3].
  • CONCLUSIONS: The simultaneous expression of two copies of gshI gene and one copy of gshII gene resulted in a significant increase in GSH production [1].


  1. Improved glutathione production by gene expression in Escherichia coli. Liao, X.Y., Shen, W., Chen, J., Li, Y., Du, G.C. Lett. Appl. Microbiol. (2006) [Pubmed]
  2. Genes Involved in Intrinsic Antibiotic Resistance of Acinetobacter baylyi. Gomez, M.J., Neyfakh, A.A. Antimicrob. Agents Chemother. (2006) [Pubmed]
  3. Cloning of a gene responsible for the biosynthesis of glutathione in Escherichia coli B. Murata, K., Kimura, A. Appl. Environ. Microbiol. (1982) [Pubmed]
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