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Gene Review

recD  -  exonuclease V (RecBCD complex), alpha chain

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2815, JW2787, hopE
 
 
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Disease relevance of recD

  • The recombination hot spot chi activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy [1].
  • Salmonella recD mutations increase recombination in a short sequence transduction assay [2].
  • We have identified recD mutants of Salmonella typhimurium by their ability to support growth of phage P22 abc (anti-RecBCD) mutants, whose growth is prevented by normal host RecBCD function [2].
  • The effect of a recipient recD mutation was eliminated if the donor P22 phage expressed its Abc (anti-RecBC) function [2].
  • Exonuclease requirements for recombination of lambda-phage in recD mutants of Escherichia coli [3].
 

High impact information on recD

  • Chi is dispensable when cells are induced for the SOS response or contain a recD mutation. recBC sbcA mutant cells are also capable of RDR induction [4].
  • Upon delivering a double-chain break to the plasmid, lambda recombined as if the cells had become recD mutants [1].
  • Two other suppressors, hopR and hopE of the same -1 frameshift mutant, cause some ribosomes reading the sequence GUG UG to decode a single amino acid, Val, from the five nucleotides [5].
  • The recB and recD subunits of the recBCD enzyme (exonuclease V) from Escherichia coli were covalently photolabeled with the ATP photoaffinity analogue [alpha-32P]8-azido-ATP [6].
  • Complete nucleotide sequence of recD, the structural gene for the alpha subunit of Exonuclease V of Escherichia coli [7].
 

Biological context of recD

  • The proposed initiation codon of the recD gene overlaps the termination codon of the upstream recB gene by one nucleotide, suggesting that these genes may form an operon [7].
  • Introducing a deletion into the nonoverlapping portion of the cloned thyA gene and inserting a gene encoding kanamycin resistance produced a (delta thyA::Kmr) that was easily transferred to the chromosome of a recD host by marker replacement [8].
  • Trans-complementation and freshly targeted gene disruption studies reconfirmed that the inactivation of the recD gene leads to a cold-sensitive phenotype [9].
  • The inactivation of recD in P. syringae also led to cell death and accumulation of DNA fragments of approximately 25-30 kbp in size at low temperature (4 degrees ) [9].
  • Transposon mutagenesis was carried out to isolate mutant(s) of the bacterium that are defective for growth at 4 degrees but normal at 22 degrees . In one such cold-sensitive mutant (CS1), the transposon-disrupted gene was identified to be a homolog of the recD gene of several bacteria [9].
 

Associations of recD with chemical compounds

 

Other interactions of recD

  • Mutation of recN reduced sectored recombinants in these crosses to approximately 1% in all the strains examined, including recD [11].
  • In wild-type cells, induction of recA expression by naladixic acid required the recBC, but not the recD function of the RecBCD enzyme [12].

References

  1. The recombination hot spot chi activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy. Myers, R.S., Kuzminov, A., Stahl, F.W. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  2. Salmonella recD mutations increase recombination in a short sequence transduction assay. Miesel, L., Roth, J.R. J. Bacteriol. (1994) [Pubmed]
  3. Exonuclease requirements for recombination of lambda-phage in recD mutants of Escherichia coli. Dermić, D., Zahradka, D., Petranović, M. Genetics (2006) [Pubmed]
  4. DNA replication triggered by double-stranded breaks in E. coli: dependence on homologous recombination functions. Asai, T., Bates, D.B., Kogoma, T. Cell (1994) [Pubmed]
  5. Mutants of translational components that alter reading frame by two steps forward or one step back. Falahee, M.B., Weiss, R.B., O'Connor, M., Doonan, S., Gesteland, R.F., Atkins, J.F. J. Biol. Chem. (1988) [Pubmed]
  6. Photoaffinity labeling of the recBCD enzyme of Escherichia coli with 8-azidoadenosine 5'-triphosphate. Julin, D.A., Lehman, I.R. J. Biol. Chem. (1987) [Pubmed]
  7. Complete nucleotide sequence of recD, the structural gene for the alpha subunit of Exonuclease V of Escherichia coli. Finch, P.W., Storey, A., Brown, K., Hickson, I.D., Emmerson, P.T. Nucleic Acids Res. (1986) [Pubmed]
  8. A transcription terminator in the thymidylate synthase (thyA) structural gene of Escherichia coli and construction of a viable thyA::Kmr deletion. Bell-Pedersen, D., Galloway Salvo, J.L., Belfort, M. J. Bacteriol. (1991) [Pubmed]
  9. RecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W. Regha, K., Satapathy, A.K., Ray, M.K. Genetics (2005) [Pubmed]
  10. Genes Involved in Intrinsic Antibiotic Resistance of Acinetobacter baylyi. Gomez, M.J., Neyfakh, A.A. Antimicrob. Agents Chemother. (2006) [Pubmed]
  11. Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr x F- crosses. Lloyd, R.G., Buckman, C. Genetics (1995) [Pubmed]
  12. Expression of the recA gene in recombination-deficient (rec-) strains of Escherichia coli. Chua, K.L., Mak, Y.K., Oliver, P. Biochimie (1993) [Pubmed]
 
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