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Gene Review

aroG  -  3-deoxy-D-arabino-heptulosonate-7...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0743, JW0737
 
 
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Disease relevance of aroG

 

High impact information on aroG

  • There is significant identity in the nucleotide sequences of aroG and aroH (the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (trp), indicating that these two genes have evolved from a common ancestral gene [1].
  • To validate our theoretical results, we performed site-directed mutagenesis to reduce the degree of DNA curvature in the regulatory sequences of the aroG, pyrC, and argCBH operons [2].
  • In vitro chemical mutagenesis of the cloned aroG gene was used to identify residues and regions of the polypeptide essential for phenylalanine feedback inhibition [3].
  • A mutation to site A of the ATP-binding site and other mutations in this region affect tyrosine-mediated repression but do not prevent activation or phenylalanine-mediated repression of aroG [4].
  • Mutations in the aroG regulatory DNA were isolated by site-directed mutagenesis and cloned in a low-copy-number plasmid to generate aroG-lac fusions [5].
 

Chemical compound and disease context of aroG

  • The promoter, operator, and 5' and 3' ends of the mRNA of the Escherichia coli gene aroG (encoding the phenylalanine-sensitive 3-deoxy-arabinoheptulosonate-7-phosphate synthase) were located [5].
 

Biological context of aroG

  • The location of the aroG promoter was identified in both strands of the DNA by in vitro DNase I footprinting and methylation protection experiments with RNA polymerase [5].
 

Analytical, diagnostic and therapeutic context of aroG

  • Here we describe the PCR amplification, cloning, and sequencing of aroG structural gene from M. tuberculosis H37Rv [6].

References

  1. The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12. Davies, W.D., Davidson, B.E. Nucleic Acids Res. (1982) [Pubmed]
  2. Genome analysis of Escherichia coli promoter sequences evidences that DNA static curvature plays a more important role in gene transcription than has previously been anticipated. Olivares-Zavaleta, N., Jáuregui, R., Merino, E. Genomics (2006) [Pubmed]
  3. Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. Kikuchi, Y., Tsujimoto, K., Kurahashi, O. Appl. Environ. Microbiol. (1997) [Pubmed]
  4. A genetic analysis of various functions of the TyrR protein of Escherichia coli. Yang, J., Ganesan, S., Sarsero, J., Pittard, A.J. J. Bacteriol. (1993) [Pubmed]
  5. Identification of the promoter, operator, and 5' and 3' ends of the mRNA of the Escherichia coli K-12 gene aroG. Baseggio, N., Davies, W.D., Davidson, B.E. J. Bacteriol. (1990) [Pubmed]
  6. DAHP synthase from Mycobacterium tuberculosis H37Rv: cloning, expression, and purification of functional enzyme. Rizzi, C., Frazzon, J., Ely, F., Weber, P.G., da Fonseca, I.O., Gallas, M., Oliveira, J.S., Mendes, M.A., de Souza, B.M., Palma, M.S., Santos, D.S., Basso, L.A. Protein Expr. Purif. (2005) [Pubmed]
 
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