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Gene Review:

purU - formyltetrahydrofolate hydrolase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1227, JW1220, tgs, ychI

Bösl, M. et al., Nagy, P.L. et al., Nixon, A.E. et al.

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Disease relevance of purU

A gene designated purU has been identified and characterized. purU is adjacent to tyrT at min 27.7 on the Escherichia coli chromosome [1].

High impact information on purU

The enzymes encoded by the Escherichia coli genes purU and purN, N10-formyltetrahydrofolate hydrolase and glycinamide ribonucleotide (GAR) transformylase, respectively, catalyze similiar yet distinct reactions [2].
A plasmid encoding ORF32 totally complemented the inability of strain DTC274 to grow on glucose minimal medium as well as the transient glycine starvation phenomenon in DTK-12, and ORF32 was designated tgs [3].
Partial deletion of tgs, cotransduced with the marker delta tyrT::kan, was responsible for the completely different phenotypes of the deletion mutants DTK-12 and DTC274 [3].
Primer extension mapping and assays of lacZ in a promoter probe vector identified two promoters giving mono- and bi-cistronic purU mRNA [1].

References

purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis. Nagy, P.L., McCorkle, G.M., Zalkin, H. J. Bacteriol. (1993) [Pubmed]
Assembly of an active enzyme by the linkage of two protein modules. Nixon, A.E., Warren, M.S., Benkovic, S.J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
Organization and functions of genes in the upstream region of tyrT of Escherichia coli: phenotypes of mutants with partial deletion of a new gene (tgs). Bösl, M., Kersten, H. J. Bacteriol. (1994) [Pubmed]

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