Disease relevance of rluA

• The Escherichia coli gene rluA, coding for the pseudouridine synthase RluA that forms 23 S rRNA pseudouridine 746 and tRNA pseudouridine 32, was deleted in strains MG1655 and BL21/DE3 [1].
• Involvement of LuxR, a quorum sensing regulator in Vibrio harveyi, in the promotion of metabolic genes: argA, purM, lysE and rluA [2].

High impact information on rluA

• Plasmid rescue of both rluA- strains using an rluA gene carrying asparagine or threonine replacements for the highly conserved aspartate 64 demonstrated that neither mutant could form 23 S RNA pseudouridine 746 or tRNA pseudouridine 32 in vivo, showing that this conserved aspartate is essential for enzyme-catalyzed formation of both pseudouridines [1].
• The four luxR-dependent promoters were upstream of argA, purM, lysE, and rluA, genes involved in arginine and purine biosyntheses, amino acid efflux, and pseudouridine synthesis, respectively [2].

Biological context of rluA

• Replacement of rluA in trans on a rescue plasmid restored both pseudouridines [1].

Associations of rluA with chemical compounds

• The rluA deletion mutant failed to form either 23 S RNA pseudouridine 746 or tRNA pseudouridine 32 [1].

References