Disease relevance of lysP

• The product of rocE shares similarity with the product of B. subtilis rocC and with the product of E. coli lysP [1].
• A chromosomal fragment encoding cadmium resistance was cloned from Pseudomonas putida 06909, a rhizosphere bacterium, and sequence analysis revealed two divergently transcribed genes, cadA and cadR [2].
• The ArcD protein of P. aeruginosa, like the LysI lysine transporter of Corynebacterium glutamicum, has 13 hydrophobic regions which could span the cytoplasmic membrane [3].

High impact information on lysP

• The cadR gene was inferred to encode an AraC/XylS type of transcriptional regulator from its deduced amino acid sequence [4].
• The cadA promoter was responsive to Cd(II), Pb(II), and Zn(II), while the cadR promoter was only induced by Cd(II) [2].
• Transcription of lysP (encodes a lysine transporter) was also examined, since it is a negative regulator of cadBA expression in the absence of lysine. lysP expression was repressed by lysine but not influenced by pH [5].
• Expression of cadA-lacZ in a strain carrying the cadC310 allele, however, was not affected by the plasmid-expressed lysP [6].
• The lysP-phoA fusion was cloned and used as a probe to clone the wild-type lysP gene [7].

Biological context of lysP

• Cells carrying a plasmid with the lysP gene exhibited a 10- to 20-fold increase in the rate of lysine uptake above wild-type levels [7].
• This lysP mutation was mapped to about 46.5 min on the E. coli chromosome [7].

Associations of lysP with chemical compounds

• Three other mutants had mutations mapping in or near lysP (cadR), a gene encoding a lysine transport protein that has previously been shown to regulate cadA expression [6].
• Mutational analysis indicated that cadA and cadR are fully responsible for cadmium resistance and partially for zinc resistance [2].

Analytical, diagnostic and therapeutic context of lysP

• Site-directed mutagenesis was also used to generate additional blaM fusions at specific locations in the lysP gene [8].

References