Disease relevance of glk

• Molecular characterization of glucokinase from Escherichia coli K-12 [1].
• Cloning and characterization of the glucokinase gene of Brucella abortus 19 and identification of three other genes [2].
• Use of heterologously expressed Zymomonas mobilis glf-encoded glucose facilitator and glk-encoded glucokinase resulted in the synthesis in 48 h of 3-dehydroshikimic acid (60 g/L) and shikimate pathway byproducts in a total yield of 41% (mol/mol) [3].

High impact information on glk

• Here we show that Mlc(Tth) is not a glucokinase but is indeed able to bind glucose (K(D) = 20 microM), unlike Mlc(Eco) [4].
• Overexpression of glk interfered with the expression of the maltose system [1].
• Open reading frames similar to E. coli mepA, glk, and genes encoding ATP-coupled exporters were found in the sequence [2].
• The edd and glk genes are separated by 203 bp [5].
• Mutations to loss of glucokinase, glk, are between the ptsI and dsd genes [6].

Chemical compound and disease context of glk

• The highest titers and yields of shikimic acid biosynthesized from glucose in 1 L fermentor runs were achieved using E. coli SP1.lpts/pSC6.090B, which expressed both Z. mobilis glf-encoded glucose facilitator protein and Z. mobilis glk-encoded glucose kinase in a host deficient in the phosphoenolpyruvate:carbohydrate phosphotransferase system [7].
• Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase [6].

Biological context of glk

• In contrast, no increase in specific growth rate (mu) was observed in VH32 with a plasmid expressing only glk from the same promoter [8].
• The purpose of this work was to study the effect of different gene expression levels, of galactose permease (GalP) and glucokinase (Glk), on glucose internalization and phosphorylation in a E. coli PTS(-) strain [8].

Associations of glk with chemical compounds

• Under all conditions tested, only growth on glucose reduced the expression of glk by about 50% [1].

Other interactions of glk

• Strains transformed with part of the plasmid family, containing both galP and glk genes, showed a mu value similar to that of W3110 [8].

References