Disease relevance of edd

• Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway [1].
• Multicopy plasmid suppression of stationary phase chaperone toxicity in Escherichia coli by phosphogluconate dehydratase and the N-terminus of DnaK [2].

High impact information on edd

• The gene is present in a single copy on the Z. mobilis genome and is not tightly linked to the edd gene [3].
• E. coli F-18 edd eda and E. coli K-12 edd eda double mutants colonized the streptomycin-treated intestine when fed to mice alone; however, when fed simultaneously with their respective parental strains, they were poor colonizers [4].
• Since the edd gene is involved only in gluconate metabolism via the Entner-Doudoroff pathway, these results implicate the utilization of gluconate and the Entner-Doudoroff pathway as important elements in E. coli colonization of the streptomycin-treated mouse large intestine [4].
• The nucleotide sequence of the entire Escherichia coli edd-eda region that encodes the enzymes of the Entner-Doudoroff pathway was determined [5].
• The edd reading frame is 1,809 bases long and encodes the 602-amino-acid, 64,446-Da protein 6-phosphogluconate dehydratase [5].

Chemical compound and disease context of edd

• When E. coli is grown on gluconate, the edd and eda genes are cotranscribed [5].

Biological context of edd

• Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon [5].
• Even though the specific growth rates between the strain carrying the edd gene knockout and its parent JM101 and PTS- Glc+ edd and its parent PTS- Glc+ were very similar, reproducible changes in the specific consumption rates and biomass yields were obtained when grown on glucose [6].

Anatomical context of edd

• Other proteins affected by the reduced GroE level included two proteins (enzymes of the Entner-Doudoroff pathway) encoded by the edd-eda operon and the ribosomal protein S6, suggesting that the GroE chaperones are involved in regulating expression of genes for carbohydrate metabolism and in modulating biogenesis or function of the ribosome [7].

Associations of edd with chemical compounds

• The edd (6-phosphogluconate dehydrase gene)-disrupted mutant accumulated 2.5 g/l of inosine from 48 g/l of sodium gluconate, compared with 1.4 g/l of inosine in the edd wild strain [8].

Other interactions of edd

• The edd and glk genes are separated by 203 bp [9].
• In order to advance in this respect, the phage lambda placMu53 was used to select operon gnt::lacZ fusion in a E. coli strain delta (edd-zwf), delta (gnd-his), delta lac [10].

References