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Gene Review:

rsmB - 16S rRNA m(5)C967 methyltransferase, SAM...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3275, JW3250, fmu, fmv, rrmB, ...

Andersen, N.M. et al., Liu, Y. et al., Liu, Y. et al., Cui, Y. et al., Ma, W. et al., et al.

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Disease relevance of rsmB

Further, fmu disruption did not significantly alter the growth rate of E. coli in rich or minimal media [1].
Indeed, the expression rsmB' DNA leads to neutralization of the negative effects of the RNA-binding protein, RsmA, in Ecc71 and Serratia marcescens strain SM274 [2].
To determine the occurrence of functional homologs of rsmB(Ecc) in non-soft-rot-causing Erwinia species, we cloned the rsmB genes of E. amylovora (rsmB(Ea)) and E. herbicola pv. gypsophilae (rsmB(Ehg)) [3].
The expression of lacZ transcriptional fusions of rsmB of Erwinia amylovora and Erwinia herbicola pv. gypsophilae was markedly reduced in a GacA- and a GacS- mutant of Pseudomonas syringae pv. syringae [4].

High impact information on rsmB

By contrast, a plasmid with the rsmB' DNA sequence deleted fails to cause overproduction of the extracellular enzymes in Ecc71 [2].
BLAST searches of the E.coli genome revealed a single gene, yebU, with sufficient similarity to rsmB to encode a putative m5C RNA methyltransferase [5].
The gene is a fusion of two open reading frames, fmu and fmv, previously believed to be distinct due to a DNA sequencing error [6].
RsmA is a small RNA-binding protein, which acts by lowering the half-life of a mRNA species. rsmB specifies an untranslated regulatory RNA and neutralizes the RsmA effect [4].
Analysis of lacZ transcriptional fusions shows that the KdgR-binding sites negatively affect the expression of rsmB [7].

Biological context of rsmB

Alignment of the protected sequences revealed the 21-mer consensus sequence of the KdgREcc-binding site as 5'-G/AA/TA/TGAAA[N6]TTTCAG/TG/TA-3'. Two such KdgREcc-binding sites occur in rsmB DNA in a close proximity to each other within nucleotides +79 and +139 and the third KdgREcc-binding site within nucleotides +207 and +231 [7].

Analytical, diagnostic and therapeutic context of rsmB

6His-RsmA recombinant protein purified from E. coli binds rsmB RNA as indicated by gel mobility shift assays. rsmB comprises 547 bp DNA, which is transcribed from a single start site immediately after a sigma70-like promoter [2].

References

Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli. Gu, X.R., Gustafsson, C., Ku, J., Yu, M., Santi, D.V. Biochemistry (1999) [Pubmed]
Characterization of a novel RNA regulator of Erwinia carotovora ssp. carotovora that controls production of extracellular enzymes and secondary metabolites. Liu, Y., Cui, Y., Mukherjee, A., Chatterjee, A.K. Mol. Microbiol. (1998) [Pubmed]
Molecular characterization of global regulatory RNA species that control pathogenicity factors in Erwinia amylovora and Erwinia herbicola pv. gypsophilae. Ma, W., Cui, Y., Liu, Y., Dumenyo, C.K., Mukherjee, A., Chatterjee, A.K. J. Bacteriol. (2001) [Pubmed]
Effects of the two-component system comprising GacA and GacS of Erwinia carotovora subsp. carotovora on the production of global regulatory rsmB RNA, extracellular enzymes, and harpinEcc. Cui, Y., Chatterjee, A., Chatterjee, A.K. Mol. Plant Microbe Interact. (2001) [Pubmed]
YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407. Andersen, N.M., Douthwaite, S. J. Mol. Biol. (2006) [Pubmed]
Purification, cloning, and characterization of the 16S RNA m5C967 methyltransferase from Escherichia coli. Tscherne, J.S., Nurse, K., Popienick, P., Michel, H., Sochacki, M., Ofengand, J. Biochemistry (1999) [Pubmed]
kdgREcc negatively regulates genes for pectinases, cellulase, protease, HarpinEcc, and a global RNA regulator in Erwinia carotovora subsp. carotovora. Liu, Y., Jiang, G., Cui, Y., Mukherjee, A., Ma, W.L., Chatterjee, A.K. J. Bacteriol. (1999) [Pubmed]

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