Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

glgB - 1,4-alpha-glucan branching enzyme

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3418, JW3395

Romeo, T. et al., Lim, W.J. et al., Garg, S.K. et al.

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Disease relevance of glgB

The nucleotide sequences of the Escherichia coli genome between the glycogen biosynthetic genes glgB and glgC, and 1170 bp of DNA which follows glgA have been determined [1].
Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35 [2].
A single glgB gene Rv1326c is present in Mycobacterium tuberculosis [3].

High impact information on glgB

The region between glgB and glgC contains an open reading frame (ORF) of 1521 bp which we call glgX [1].
The deduced amino acid (aa) sequence for the putative product shows significant similarity to the E. coli glycogen branching enzyme, and to several different glucan hydrolases and transferases [1].

Analytical, diagnostic and therapeutic context of glgB

The glycogen branching enzyme (GlgB) was purified and the molecular weight of the enzyme was estimated to be 84kDa by SDS-PAGE [2].

References

Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes. Romeo, T., Kumar, A., Preiss, J. Gene (1988) [Pubmed]
Cloning and characterization of the glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme from Pectobacterium chrysanthemi PY35. Lim, W.J., Park, S.R., Kim, M.K., An, C.L., Yun, H.J., Hong, S.Y., Kim, E.J., Shin, E.C., Lee, S.W., Lim, Y.P., Yun, H.D. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
Expression and characterization of alpha-(1,4)-glucan branching enzyme Rv1326c of Mycobacterium tuberculosis H37Rv. Garg, S.K., Alam, M.S., Kishan, K.V., Agrawal, P. Protein Expr. Purif. (2007) [Pubmed]

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