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Gene Review

glgC  -  glucose-1-phosphate adenylyltransferase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3416, JW3393
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Disease relevance of glgC


High impact information on glgC

  • By mutagenizing the LS cDNA with hydroxylamine and then coexpressing with wild-type SS in an E. coli glgC- strain, >350 mutant colonies were identified that were impaired in glycogen production [4].
  • ADPglucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase; ADP:alpha-D-glucose-1-phosphate adenylyltransferase, EC catalyzes a key regulatory step in alpha-glucan synthesis in bacteria and higher plants [4].
  • A potato (Solanum tuberosum L.) AGPase LS down-regulatory mutant (E38A) was subjected to random mutagenesis using error-prone polymerase chain reaction and screened for the capacity to form an enzyme capable of restoring glycogen production in glgC(-) Escherichia coli [5].
  • In all of the metabolic conditions studied in this report the adenylate energy charge ((ATP + 1/2 ADP)/(ATP + ADP + AMP)) and the level of the rate-limiting enzyme of glycogen synthesis, ADP-glucose synthetase (glucose 1-phosphate adenylyltransferase, EC, were the same [6].
  • Results from toeprint and cell-free translation experiments indicate that bound CsrA prevents ribosome binding to the glgC Shine-Dalgarno sequence and that this reduces GlgC synthesis [7].

Biological context of glgC

  • The carbon storage regulator gene, csrA, encodes a factor which negatively modulates the expression of the glycogen biosynthetic gene glgC by enhancing the decay of its mRNA (M. Y. Liu, H. Yang, and T. Romeo, J. Bacteriol. 177:2663-2672, 1995) [8].
  • The nucleotide sequences of the Escherichia coli genome between the glycogen biosynthetic genes glgB and glgC, and 1170 bp of DNA which follows glgA have been determined [9].
  • The region between glgB and glgC contains an open reading frame (ORF) of 1521 bp which we call glgX [9].
  • The structural gene, glgC, for the allosteric mutant enzyme was selected by colony hybridization and cloned into the bacterial plasmid pBR322 by insertion of the chromosomal DNA at the PstI site [10].
  • One recombinant plasmid, designated pKG3, was isolated from the genomic library of CL1136 containing glgC [10].

Associations of glgC with chemical compounds


  1. Cloning and sequencing of glycogen metabolism genes from Rhodobacter sphaeroides 2.4.1. Expression and characterization of recombinant ADP-glucose pyrophosphorylase. Igarashi, R.Y., Meyer, C.R. Arch. Biochem. Biophys. (2000) [Pubmed]
  2. Comparison of the 5' flanking regions of the Salmonella typhimurium and Escherichia coli glgC genes, encoding ADP glucose pyrophosphorylases. Romeo, T., Moore, J. Nucleic Acids Res. (1991) [Pubmed]
  3. Cloning and expression of the glgC gene from Agrobacterium tumefaciens: purification and characterization of the ADPglucose synthetase. Uttaro, A.D., Ugalde, R.A., Preiss, J., Iglesias, A.A. Arch. Biochem. Biophys. (1998) [Pubmed]
  4. Mutagenesis of the potato ADPglucose pyrophosphorylase and characterization of an allosteric mutant defective in 3-phosphoglycerate activation. Greene, T.W., Chantler, S.E., Kahn, M.L., Barry, G.F., Preiss, J., Okita, T.W. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  5. Analysis of allosteric effector binding sites of potato ADP-glucose pyrophosphorylase through reverse genetics. Kavakli, I.H., Park, J.S., Slattery, C.J., Salamone, P.R., Frohlick, J., Okita, T.W. J. Biol. Chem. (2001) [Pubmed]
  6. Contribution of cyclic adenosine 3':5'-monophosphate to the regulation of bacterial glycogen synthesis in vivo. Effect of carbon source and cyclic adenosine 3':5'-monophosphate on the quantitative relationship between the rate of glycogen synthesis and the cellular concentrations of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli. Dietzler, D.N., Leckie, M.P., Magnani, J.L., Sughrue, M.J., Bergstein, P.E., Sternheim, W.L. J. Biol. Chem. (1979) [Pubmed]
  7. CsrA regulates glycogen biosynthesis by preventing translation of glgC in Escherichia coli. Baker, C.S., Morozov, I., Suzuki, K., Romeo, T., Babitzke, P. Mol. Microbiol. (2002) [Pubmed]
  8. Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product. Yang, H., Liu, M.Y., Romeo, T. J. Bacteriol. (1996) [Pubmed]
  9. Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes. Romeo, T., Kumar, A., Preiss, J. Gene (1988) [Pubmed]
  10. Cloning, expression, and nucleotide sequence of glgC gene from an allosteric mutant of Escherichia coli B. Ghosh, P., Meyer, C., Remy, E., Peterson, D., Preiss, J. Arch. Biochem. Biophys. (1992) [Pubmed]
  11. Identification and molecular characterization of csrA, a pleiotropic gene from Escherichia coli that affects glycogen biosynthesis, gluconeogenesis, cell size, and surface properties. Romeo, T., Gong, M., Liu, M.Y., Brun-Zinkernagel, A.M. J. Bacteriol. (1993) [Pubmed]
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