Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

metJ - transcriptional repressor, S...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3930, JW3909

Smith, A.A. et al., Saint-Girons, I. et al., Shoeman, R. et al., Usuda, Y. et al., Marincs, F. et al., et al.

Usuda, Kurahashi,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of metJ

Isolation and characterization of the product of the methionine-regulatory gene metJ of Escherichia coli K-12 [1].

High impact information on metJ

The subunit size and the amino acid composition are the same as those predicted from the DNA sequence of the metJ gene [1].
Bacterial strains carrying this plasmid produce elevated levels of the metJ gene product, presumably because of the high number of gene copies in the cell [1].
Structure and autoregulation of the metJ regulatory gene in Escherichia coli [2].
Analysis of the sequence downstream from the metJ gene has revealed inverted repeats homologous to several intercistronic regions, also reported to occur between operons [2].
Three of the mutations in the metK structural gene were then introduced into metJ and thrBC double-mutant strains; one of the resultant strains was found to accumulate 0.13 g/liter Met [3].

Biological context of metJ

Evidence was provided for autoregulation by repression of the metJ gene [2].
In a simplified system that measures the formation of the first dipeptide of the gene product (fMet-Ala for the metJ gene), MetJ protein and AdoMet partially repress (approximately 40-60%) metJ gene expression [4].

Associations of metJ with chemical compounds

Expression of methionine regulon elements was controlled by the metJ protein gpMetJ [5].

Analytical, diagnostic and therapeutic context of metJ

We have used DNA arrays to investigate the effects of knocking out the methionine repressor gene, metJ, on the Escherichia coli transcriptome [6].

References

Isolation and characterization of the product of the methionine-regulatory gene metJ of Escherichia coli K-12. Smith, A.A., Greene, R.C., Kirby, T.W., Hindenach, B.R. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
Structure and autoregulation of the metJ regulatory gene in Escherichia coli. Saint-Girons, I., Duchange, N., Cohen, G.N., Zakin, M.M. J. Biol. Chem. (1984) [Pubmed]
Effects of deregulation of methionine biosynthesis on methionine excretion in Escherichia coli. Usuda, Y., Kurahashi, O. Appl. Environ. Microbiol. (2005) [Pubmed]
Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the in vitro expression of the metB, metL and metJ genes. Shoeman, R., Coleman, T., Redfield, B., Greene, R.C., Smith, A.A., Saint-Girons, I., Brot, N., Weissbach, H. Biochem. Biophys. Res. Commun. (1985) [Pubmed]
Control of metF gene expression in maxicell preparations of Escherichia coli K-12: reversible action of the metJ protein and effect of vitamin B12. Emmett, M.R., Johnson, J.R. J. Bacteriol. (1986) [Pubmed]
Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor. Marincs, F., Manfield, I.W., Stead, J.A., McDowall, K.J., Stockley, P.G. Biochem. J. (2006) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.