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Gene Review:

metA - homoserine O-transsuccinylase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4005, JW3973

Lamonte, B.L. et al., Michaeli, S. et al., Röhl, F. et al., Bourhy, P. et al., Gery, S. et al., et al.

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Disease relevance of metA

Regulatory region of the metA gene of Escherichia coli K-12 [1].
PBSX, a defective Bacillus subtilis prophage, maps to the metA-metC region of the chromosome [2].

High impact information on metA

Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli [3].
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase [4].
The Leptospira meyeri serovar semaranga metX gene was identified by complementation of an Escherichia coli metA mutant, i.e., devoid of homoserine O-succinyltransferase [5].
However, in the metA gene there is a second transcription start point which is located 74 base pairs upstream and which is independent of the intracellular methionine concentration [1].
Cultures with in vivo SAMase activity were assayed for expression of the metA, B, C, E, F, H, J, K and R genes in cells grown in methionine-rich complete media as well as in defined media with and without l-methionine [6].

Chemical compound and disease context of metA

The molecular basis for growth inhibition of Escherichia coli was a non-competitive inhibition of homoserine-O-succinyltransferase (EC 2.3.1.46), thus blocking methionine biosynthesis [7].

Other interactions of metA

We have identified by lacZ fusion an additional promoter in this region, and showed that it is transcribed in the opposite orientation from the metA gene [8].

References

Regulatory region of the metA gene of Escherichia coli K-12. Michaeli, S., Mevarech, M., Ron, E.Z. J. Bacteriol. (1984) [Pubmed]
Characterization of PBSX, a defective prophage of Bacillus subtilis. Wood, H.E., Dawson, M.T., Devine, K.M., McConnell, D.J. J. Bacteriol. (1990) [Pubmed]
Nucleotide sequence of the metA gene encoding homoserine trans-succinylase in Escherichia coli. Duclos, B., Cortay, J.C., Bleicher, F., Ron, E.Z., Richaud, C., Saint Girons, I., Cozzone, A.J. Nucleic Acids Res. (1989) [Pubmed]
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase. Born, T.L., Blanchard, J.S. Biochemistry (1999) [Pubmed]
Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited. Bourhy, P., Martel, A., Margarita, D., Saint Girons, I., Belfaiza, J. J. Bacteriol. (1997) [Pubmed]
In vivo hydrolysis of S-adenosylmethionine induces the met regulon of Escherichia coli. Lamonte, B.L., Hughes, J.A. Microbiology (Reading, Engl.) (2006) [Pubmed]
Biological properties and mode of action of clavams. Röhl, F., Rabenhorst, J., Zähner, H. Arch. Microbiol. (1987) [Pubmed]
An Escherichia coli gene divergently transcribed from a promoter overlapping the metA promoter. Gery, S., Ron, E.Z. FEMS Microbiol. Lett. (1997) [Pubmed]

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