The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

aphA  -  acid phosphatase/phosphotransferase, class...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4047, JW4015, hobH, napA, yjbP
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of aphA

  • Indeed, the expression of a chromosomal tcpP-lacZ fusion was reduced in the delta aphA mutant and increased in both V. cholerae and Escherichia coli by introducing aphA expressed from an inducible promoter [1].
  • We describe here a previously unidentified gene in Vibrio cholerae, aphA (activator of tcpP and tcpH expression), which is required for the transcription of the tcpPH operon [1].
  • The aphA gene of Salmonella enterica sv [2].

High impact information on aphA

  • Under conditions normally optimal for virulence gene expression, an in frame aphA deletion decreased the expression of a cholera toxin promoter fusion (ctx-lacZ) and prevented the production of the toxin co-regulated pilus (TCP) [1].
  • Plasmids producing ToxT or TcpP/H, but not ToxR, restored ctx-lacZ expression and TCP production in the delta aphA strain, suggesting that the mutation interferes with toxT expression by influencing the transcription of tcpPH [1].
  • (a) aphA was overexpressed in Escherichia coli with N-terminal His and S tags, and the protein was reconstituted by an optimized protocol with phycocyanobilin (PCB), to yield the photochromic chromoprotein, PCB-AphA, carrying the PCB chromophore [3].
  • (d) Through deletion mutations of aphA, a short sequence IQPHGV [amino acids (aa) 26-31] was found essential for the lyase activity of AphA, indicating an interaction of the N terminus with the chromophore-binding domain around cysteine 259 [3].


  1. A new level in the Vibrio cholerae ToxR virulence cascade: AphA is required for transcriptional activation of the tcpPH operon. Skorupski, K., Taylor, R.K. Mol. Microbiol. (1999) [Pubmed]
  2. Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium. Makde, R.D., Kumar, V., Gupta, G.D., Jasti, J., Singh, T.P., Mahajan, S.K. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
  3. Photochromic biliproteins from the cyanobacterium Anabaena sp. PCC 7120: lyase activities, chromophore exchange, and photochromism in phytochrome AphA. Zhao, K.H., Ran, Y., Li, M., Sun, Y.N., Zhou, M., Storf, M., Kupka, M., Böhm, S., Bubenzer, C., Scheer, H. Biochemistry (2004) [Pubmed]
WikiGenes - Universities