Disease relevance of leuX

• Growth competition in CD-1 mouse mucus between the wild-type strain E. coli 536, its leuX mutant 536 delta 102 and its mutant 536R3, lacking both pathogenicity islands but expressing a functional tRNA5(Leu), revealed a major impact of leuX on E. coli survival in bladder mucus [1].
• The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNALeu isoacceptors of E. coli and with two tRNALeu species from bacteriophage T4 and T5 [2].

High impact information on leuX

• The leuX (supP) gene of Escherichia coli codes for a suppressor tRNA (tRNA(6Leu] that inserts leucine at the amber codon [3].
• An integrase with strong homology to the LEE O26 integrase was detected on the K-12 chromosome associated with the leuX tRNA locus at 97 min [4].
• Interestingly, the archetype E. coli K1 neonatal meningitis strain, strain RS218, has two copies of the dsdCXA genes for d-serine utilization at the argW and leuX islands [5].
• We describe the cloning and the DNA sequence of an amber suppressor allele of the Escherichia coli leuX (supP) gene [2].
• Mapping of the supP (Su6+) amber suppressor gene in Escherichia coli [6].

Chemical compound and disease context of leuX

• Role of leuX in Escherichia coli colonization of the streptomycin-treated mouse large intestine [7].

Associations of leuX with chemical compounds

• In the present investigation we show that the gene responsible for stimulation of type 1 fimbriae synthesis appears to be leuX, which encodes a tRNA specific for the rare leucine codon UUG [8].

References