Disease relevance of endA

• Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome [1].
• Heterologous expression of an endoglucanase gene (endA) from the ruminal anaerobe Ruminococcus flavefaciens 17 in Streptococcus bovis and Streptococcus sanguis [2].
• The endA gene encoding endonuclease I from the psychrophilic and mildly halophilic bacterium Vibrio salmonicida and from the mesophilic brackish water bacterium Vibrio cholerae have been cloned, over-expressed in Escherichia coli, and purified [3].

High impact information on endA

• A cold-shock supernatant from cells harbouring a multicopy endA+ plasmid contained an approx. tenfold higher amount than wild-type cells of the DNA double-strand- and single-strand-cleavage activities characteristic of EndoI [4].
• S. bovis JB1 containing the endA gene was capable of utilizing cellotetraose at a faster rate than the parent strain [2].
• The genome sequence revealed that it possesses two open reading frames (ORFs) that are homologous to endA, a protein responsible for splicing endonuclease activity in archaea [5].

References