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Gene Review:

galE - UDP-glucose 4-epimerase

Haemophilus influenzae Rd KW20

Jennings, M.P. et al., Maskell, D.J. et al., Maskell, D.J. et al.

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Disease relevance of galE

Cloning and molecular analysis of the galE gene of Neisseria meningitidis and its role in lipopolysaccharide biosynthesis [1].
A deletion/insertion mutation lacking the 3' end of ORF1, all of galE, and the 5' end of ORF3 was constructed in the parent Hib strain (RM7004) [2].
The galE gene from Haemophilus influenzae was used as a hybridization probe for the galE gene of Neisseria meningitidis Group B, identifying two different homologous loci [1].

High impact information on galE

Analysis of the LPS from the galE mutant strain revealed an apparent reduction in molecular weight and a loss of reactivity with monoclonal antibodies specific for structures known to contain galactose [1].
These results are consistent with an essential role for galE in the incorporation of galactose into meningococcal lipopolysaccharide [1].
Each of the loci was cloned and nucleotide sequence analysis revealed that both loci contained sequences similar to galE [1].
These mutants had a galE phenotype, as evidenced by galactose sensitivity, altered LPS when grown in the absence of exogenous galactose, and reduced virulence in infant rats [2].
ORF2 is galE, encoding uridine 5'-diphosphogalactose 4-epimerase, and ORF4 is adk, encoding adenylate kinase [3].

References

Cloning and molecular analysis of the galE gene of Neisseria meningitidis and its role in lipopolysaccharide biosynthesis. Jennings, M.P., van der Ley, P., Wilks, K.E., Maskell, D.J., Poolman, J.T., Moxon, E.R. Mol. Microbiol. (1993) [Pubmed]
Molecular analysis of a complex locus from Haemophilus influenzae involved in phase-variable lipopolysaccharide biosynthesis. Maskell, D.J., Szabo, M.J., Butler, P.D., Williams, A.E., Moxon, E.R. Mol. Microbiol. (1991) [Pubmed]
Molecular biology of phase-variable lipopolysaccharide biosynthesis by Haemophilus influenzae. Maskell, D.J., Szabo, M.J., Butler, P.D., Williams, A.E., Moxon, E.R. J. Infect. Dis. (1992) [Pubmed]

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