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Gene Review:

rbcL - ribulose bisophosphate carboxylase

Synechocystis sp. PCC 6803

This record was discontinued.

Amichay, D. et al., Sakuragi, Y. et al., Amichay, D. et al.

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Disease relevance of rbcL

This vector was utilized for the construction of a cyanobacterial rbc null mutant in which the entire sequence comprising both rbc genes, was replaced by the Rhodospirillum rubrum rbcL gene linked to a chloramphenicol resistance gene [1].

High impact information on rbcL

Photosystem II activity and carboxysome synthesis are lost in the tocopherol mutants within 24 h of photomixotrophic growth, and the abundance of carboxysome gene (rbcL, ccmK1, ccmL) and ndhF4 transcripts decreases to undetectable levels [2].
Most of the rbcL gene encoding the large subunit of ribulose bisphosphate carboxylase/oxygenase (Rubisco) was duplicated in the genome by a targeted insertion of a 3'-truncated gene copy into the psb A-I locus [3].
The 3' and the 5' truncated rbcL genes were linked to chloramphenicol and kanamycin resistance markers, respectively [3].
Our results indicate extensive recombinatorial interactions between the homologous sequences at both loci leading to reconstitution of the cyanobacterial rbcL gene [3].

References

Construction of a Synechocystis PCC6803 mutant suitable for the study of variant hexadecameric ribulose bisphosphate carboxylase/oxygenase enzymes. Amichay, D., Levitz, R., Gurevitz, M. Plant Mol. Biol. (1993) [Pubmed]
alpha-Tocopherol plays a role in photosynthesis and macronutrient homeostasis of the cyanobacterium Synechocystis sp. PCC 6803 that is independent of its antioxidant function. Sakuragi, Y., Maeda, H., Dellapenna, D., Bryant, D.A. Plant Physiol. (2006) [Pubmed]
Restoration of the wild-type locus in an RuBP carboxylase/oxygenase mutant of Synechocystis PCC 6803 via targeted gene recombination. Amichay, D., Sheffer, M., Gurevitz, M. Mol. Gen. Genet. (1992) [Pubmed]

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