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Gene Review:

ndhB - NAD(P)H-quinone oxidoreductase subunit 2

Synechocystis sp. PCC 6803

This record was discontinued.

Ogawa, T., Thomas, D.J. et al., Aoki, S. et al., Cournac, L. et al., Ohkawa, H. et al., et al.

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Disease relevance of ndhB

A defined mutant (M55), constructed by inactivating the ndhB gene in wild-type Synechocystis, required high CO2 conditions for growth and was unable to transport CO2 and HCO3- into the intracellular Ci pool [1].

High impact information on ndhB

Sequencing of the insert DNA in the region of the mutation in RKa revealed an open reading frame (designated as ndhB), which showed extensive amino acid sequence homology to the subunit-2 genes of NADH dehydrogenase (EC 1.6.99.3) (ndhB) of chloroplasts and mitochondria [1].
In the ndhB mutant M55, which is defective in the type I NADPH-dehydrogenase complex (NDH-1) and produces only low amounts of O2 in the light, H2 uptake was negligible during dark-to-light transitions, allowing several minutes of continuous H2 production [2].
A dnaK-reporting bioluminescent Synechocystis strain was constructed by fusing a promoterless segment of the luxAB gene set downstream of the promoter region of the Synechocystis dnaK gene and introduction of this gene fusion into a BglII site downstream of the ndhB gene in the Synechocystis chromosome [3].
Photoinhibition and light-induced cyclic electron transport in ndhB(-) and psaE(-) mutants of Synechocystis sp. PCC 6803 [4].
The ndhB(-) mutant showed much lower rates of PSI cyclic electron transport than the wild type following brief dark adaptation but exceeded wild type rates after exposure to photoinhibitory light [4].

Chemical compound and disease context of ndhB

Light-induced dynamic changes of NADPH fluorescence in Synechocystis PCC 6803 and its ndhB-defective mutant M55 [5].

Associations of ndhB with chemical compounds

In these mutants and a mutant (M55) lacking ndhB, CO(2) uptake was much more strongly inhibited than HCO(3)(-) uptake, i.e., the activities of CO(2) and HCO(3)(-) uptake in B1 were 9 and 85% of those in the WT, respectively [6].

Analytical, diagnostic and therapeutic context of ndhB

Sequence analysis of the ndhB gene of RKa mutant revealed a G----A substitution that results in a Gly----Asp substitution in the deduced amino acid [1].

References

A gene homologous to the subunit-2 gene of NADH dehydrogenase is essential to inorganic carbon transport of Synechocystis PCC6803. Ogawa, T. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
Sustained photoevolution of molecular hydrogen in a mutant of Synechocystis sp. strain PCC 6803 deficient in the type I NADPH-dehydrogenase complex. Cournac, L., Guedeney, G., Peltier, G., Vignais, P.M. J. Bacteriol. (2004) [Pubmed]
Circadian expression of the dnaK gene in the cyanobacterium Synechocystis sp. strain PCC 6803. Aoki, S., Kondo, T., Ishiura, M. J. Bacteriol. (1995) [Pubmed]
Photoinhibition and light-induced cyclic electron transport in ndhB(-) and psaE(-) mutants of Synechocystis sp. PCC 6803. Thomas, D.J., Thomas, J., Youderian, P.A., Herbert, S.K. Plant Cell Physiol. (2001) [Pubmed]
Light-induced dynamic changes of NADPH fluorescence in Synechocystis PCC 6803 and its ndhB-defective mutant M55. Mi, H., Klughammer, C., Schreiber, U. Plant Cell Physiol. (2000) [Pubmed]
Mutation of ndh genes leads to inhibition of CO(2) uptake rather than HCO(3)(-) uptake in Synechocystis sp. strain PCC 6803. Ohkawa, H., Price, G.D., Badger, M.R., Ogawa, T. J. Bacteriol. (2000) [Pubmed]

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