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Gene Review:

sll0654 - alkaline phosphatase

Synechocystis sp. PCC 6803

This record was discontinued.

Hirani, T.A. et al., Mérida, A. et al., Suzuki, S. et al.

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Disease relevance of sll0654

Although there are three assignments for putative alkaline phosphatase genes in the Synechocystis sp. PCC 6803 genome, only sll0654 expression was detected by northern analysis under phosphate limitation [1].
An alignment identified a conserved 177 amino acid domain that was found at the N-terminus of the protein encoded by sll0654 but at the C-terminus of the protein in Synechococcus sp. PCC 7942 [1].

High impact information on sll0654

We identified previously a two component system, which consists of histidine kinase SphS and its cognate response regulator SphR, which regulates the expression of the phoA gene for alkaline phosphatase under phosphate-limiting conditions in the cyanobacterium Synechocystis sp. PCC 6803 [2].
The enzyme can be reactivated in vitro by different methods such as alkaline phosphatase treatment, but not phosphodiesterase, by raising the pH of the crude extract to values higher than 8, by increasing the ionic strength of the cell-free extract, or by preincubation with organic solvents, such as 2-propanol and ethanol [3].

References

Characterization of a two-component signal transduction system involved in the induction of alkaline phosphatase under phosphate-limiting conditions in Synechocystis sp. PCC 6803. Hirani, T.A., Suzuki, I., Murata, N., Hayashi, H., Eaton-Rye, J.J. Plant Mol. Biol. (2001) [Pubmed]
The SphS-SphR two component system is the exclusive sensor for the induction of gene expression in response to phosphate limitation in synechocystis. Suzuki, S., Ferjani, A., Suzuki, I., Murata, N. J. Biol. Chem. (2004) [Pubmed]
In vitro reactivation of in vivo ammonium-inactivated glutamine synthetase from Synechocystis sp. PCC 6803. Mérida, A., Candau, P., Florencio, F.J. Biochem. Biophys. Res. Commun. (1991) [Pubmed]

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