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Gene Review

isiB  -  flavodoxin FldA

Synechocystis sp. PCC 6803

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Disease relevance of isiB

  • Sequence of the flavodoxin-encoding gene from the cyanobacterium Synechocystis PCC6803 [1].
  • Cloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isiB and its own promoter [2].
  • The photoreduction of flavodoxin semiquinone by PSI from C. reinhardtii lacked fast first-order kinetic components and, in contrast with PSI from cyanobacteria, displayed only a single concentration-dependent phase [3].
 

High impact information on isiB

  • Membranes of all Lys106 mutants showed wild type rates of flavodoxin reduction and flavodoxin-mediated NADP+ reduction, but had 10-54% decrease in the ferredoxin-mediated NADP+ reduction rates [4].
  • In isolated wild-type membranes, the rate of flavodoxin reduction and flavodoxin-mediated NADP+ reduction were 800 and 480 mumol/mg of chlorophyll/h, respectively [5].
  • Genetic deletion of isiAB resulted in a photosensitive phenotype, with accumulation of reactive oxygen species and cell bleaching in high light, while the flavodoxin-deficient isiB null mutant expressing isiA was phototolerant [6].
  • The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions [6].
  • Thus, the inversion of charge at position 35 of PsaC negatively affects the rate of electron transfer to both forms of flavodoxin, whereas PSI complexes that retain a positive charge at this position show wild-type kinetics [3].
 

Chemical compound and disease context of isiB

 

Other interactions of isiB

 

Analytical, diagnostic and therapeutic context of isiB

  • 8. Sequence comparison based on the 42 N-terminal amino acids indicates that Synechocystis 6803 flavodoxin most likely belongs to the long-chain class, despite an apparent molecular mass of 15 kDa determined by SDS-PAGE [7].

References

  1. Sequence of the flavodoxin-encoding gene from the cyanobacterium Synechocystis PCC6803. Poncelet, M., Cassier-Chauvat, C., Chauvat, F. Gene (1994) [Pubmed]
  2. Cloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isiB and its own promoter. Bes, M.T., Hernández, J.A., Peleato, M.L., Fillat, M.F. FEMS Microbiol. Lett. (2001) [Pubmed]
  3. Lys35 of PsaC is required for the efficient photoreduction of flavodoxin by photosystem I from Chlamydomonas reinhardtii. Meimberg, K., Fischer, N., Rochaix, J.D., Mühlenhoff, U. Eur. J. Biochem. (1999) [Pubmed]
  4. Mutational analysis of photosystem I polypeptides. Role of PsaD and the lysyl 106 residue in the reductase activity of the photosystem I. Chitnis, V.P., Jungs, Y.S., Albee, L., Golbeck, J.H., Chitnis, P.R. J. Biol. Chem. (1996) [Pubmed]
  5. Mutational analysis of photosystem I polypeptides in Synechocystis sp. PCC 6803. Subunit requirements for reduction of NADP+ mediated by ferredoxin and flavodoxin. Xu, Q., Jung, Y.S., Chitnis, V.P., Guikema, J.A., Golbeck, J.H., Chitnis, P.R. J. Biol. Chem. (1994) [Pubmed]
  6. The chlorophyll-binding protein IsiA is inducible by high light and protects the cyanobacterium Synechocystis PCC6803 from photooxidative stress. Havaux, M., Guedeney, G., Hagemann, M., Yeremenko, N., Matthijs, H.C., Jeanjean, R. FEBS Lett. (2005) [Pubmed]
  7. Ferredoxin and flavodoxin from the cyanobacterium Synechocystis sp PCC 6803. Bottin, H., Lagoutte, B. Biochim. Biophys. Acta (1992) [Pubmed]
  8. Transcriptional analysis of the isiAB operon in salt-stressed cells of the cyanobacterium Synechocystis sp. PCC 6803. Vinnemeier, J., Kunert, A., Hagemann, M. FEMS Microbiol. Lett. (1998) [Pubmed]
 
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